秋水仙素对C6胶质瘤细胞葡萄糖摄取的影响

The influence of colchicine on glucose uptake by C6 glioma cells

目的观察秋水仙素对C6胶质瘤细胞葡萄糖摄取的影响。方法C6胶质瘤细胞与秋水仙素在37℃下孵育3 h后,再用含125μmol/L未标记2-脱氧葡萄糖(2-DOG)和37×103Bq[3H]2-DOG的磷酸盐缓冲液(PBS)溶液1ml室温下孵育5min。用液闪计数分析仪检测C6细胞葡萄糖摄取。结果秋水仙素以浓度依赖的方式抑制C6细胞葡萄糖摄取率7％～27％。卡巴可刺激葡萄糖摄取增加11％～24％,但这种作用可因秋水仙素的预处理而抵消。胰岛素刺激C6细胞葡萄糖摄取增加35％～47％,且刺激作用不被秋水仙素抵消。结论秋水仙素可能通过抑制微管的聚合使C6细胞基础糖摄取和卡巴可刺激的葡萄糖摄取下降。

Objective To observe the influence of colchicine on glucose uptake by C6 glioma cells. Methods C6 glioma cells were incubated with colchicine for 3 h at 37℃, and then the cells were incubated in 1 ml PBS containing 125μmol/L unlabeled 2-deoxy-D-glucose and 37 × 103 Bq [3H]2-de-oxy-D-glucose for 5 min at 37℃. By using liquid scintillation analyzer, the glucose uptake by C6 glioma cells was determined. Results Colchicine could inhibit basal glucose uptake in a concentration-dependent manner by 7 % to 27 % . Glucose uptake was increased with carbachol by 11 % to 24 % , but this action was abolished by pretreatment with colchicines. Basal glucose uptake was increased by insulin in a concentration-dependent manner by 35 % to 47 %, but this action was not affected by pretreatment with colchicines. Conclusion Colchicine is involved in basal and carbachol-stimulated glucose uptake by C6 cells.