黄芪在犬肾离体灌注保存中的作用

The effects of astragalus during hypothermia perfusion and preservation of isolated kidney

目的研究黄芪提取液对犬肾离体低温灌注及保存中缺血再灌注损伤的影响。方法以常规的HC-A保存液为对照组,以高渗枸橼酸盐嘌呤溶液(HC-A)添加黄芪提取液为实验组,分别对离体犬肾进行低温灌注保存24、48、72 h。通过光镜、电镜观察肾组织结构变化,建立犬肾移植模型,检测移植前后生化指标变化,综合分析。结果实验组保存的犬肾组织超微结构损伤明显轻于对照组。病理组织检查评分,实验组低于对照组(P<0.05)。移植肾早期功能指标检测,移植后2 h实验组血肌酐值(98.25±36.76)μmol/L明显低于对照组(147.26±59.67)μmol/L;移植后24 h血肌酐值(221.55±62.13)μmol/L明显低于对照组(316.12±74.31)μmol/L,而内生肌酐清除值实验组(1.91±0.76)ml·min-1·kg-1明显高于对照组(0.87±0.82)ml·min-1·kg-1,差异均具有显著性(P<0.05)。结论黄芪用于肾脏低温灌注保存中,对缺血再灌注损伤的肾脏有一定保护作用。

Objective To investigate the effects of astragalus during hypothermia perfusion and preservation of isolated kidneys. Methods The experimental group was given the extract of astragalus plus HC-A solution and the control group given HC-A solution only. The isolated kidneys of dogs were flused and stored at 0-2℃ for 24 h, 48 h and 72 h. The renal cortex was taken for morphology analysis and ultrastructure study from different perfusate used and different time preserved. Serum creatinine (Scr) was examined before operation and 2 h, 24 h after renal transplantation. 24-h urine was collected to determine creatinine clearance (Ccr). Results Light microscopic and electromicroscopic studies showed that histomorphological changes of kidneys in experimental group were much milder than that in control group. The value of Ccr in experimental group was significantly higher than that in control group ( P < 0. 05 ) . Scr after transplantation in experimental group was greatly lower than that in control group ( P < 0. 05 ) . Conclusion Astragalus have protective effects on hypothermia perfusion and preservation of isolated kidney.