EGFR和磷酸化酪氨酸在中耳胆脂瘤的表达情况

Expressions of EGFR and Phosphotyrosine in Human Middle Ear Cholesteatoma

目的探讨表皮生长因子受体(EGFR)和磷酸化酪氨酸在中耳继发性胆脂瘤的表达情况,分析其在胆脂瘤上皮增殖演变过程中的作用,以及两者之间可能的联系。方法应用免疫组化SP染色方法和计算机图像分析系统,检测20例胆脂瘤上皮,10例术腔内肉芽组织,10例鼓膜穿孔部位邻近皮肤和20例耳道深部正常皮肤中EGFR和磷酸化酪氨酸的表达情况。结果 EGFR和磷酸化酪氨酸在胆脂瘤上皮各层细胞均存在高度表达,以基底层和棘层为著;穿孔部位邻近皮肤则在基底层和棘层细胞中等表达;耳道深部正常皮肤仅基底层弱表达;肉芽组织内存在散在的阳性细胞。两者之间存在正相关,总相关系数为0.993(P<0.01)。结论EGFR和磷酸化酪氨酸在中耳继发性胆脂瘤上皮中的高度表达,说明胆脂瘤上皮具有高度增殖能力。在穿孔部位邻近皮肤中的中度表达,说明该处皮肤增生较活跃。

Purpose To explore the expressions of EGFR and Phosphotyrosine in acquired middle ear cholesteatoma, and to analyze the two factors playing the possible roles in proliferation ability of cholesteatoma epidermis, and the possible relationship between the two. Methods The specimens from the acquired middle ear cholesteatoma tissue of 20 cases, the granulation tissue of 10 cases, the adjacent skin around perforation of 10 cases and the normal external ear skin of 20 cases were examined by immunohistochemical S-P method and computer image analysis. Results The presence of EGFR and phosphotyrosine in all epithelial layers of cholesteatoma were abundant expressions, especially in the basal and spinous layers. The basal and spinous layers were middle expressions in the adjacent skin around perforation. Only the basal layer were slightly stained in the normal external ear skin. The granulation tissue was scatteredly stained. There was positive correlation between the two factors, and the total coefficient of correlation was 0. 993 ( P < 0. 01). Conclusion EGFR and phosphotyrosine play pivotal roles in the differentiation and the hyperproliferation of cholesteatoma. The adjacent skin around perforation has a relatively hyperproliferative ability.