进口饲料及肉制品中牛源性成分的PCR检测

Detection of bovine-derived materials in import animal feeds and food by PCR assay

建立了进口饲料及肉骨粉中牛源性成分的PCR检测方法。扩增对象是牛线粒体上特异性的DNA片段 ,扩增产物为 2 71bp。样品的PCR产物经直接测序及限制性酶切片段分析 ,以进一步鉴定扩增结果。方法灵敏度实验结果显示 ,当肉骨粉样品中含有 0 1 2 5 %的牛源性成分时仍能检出。本方法简便、快速 ,灵敏 。

A PCR assay for detection and identification of bovine-derived materials in import animal feeds and food is developed. A 271 bp sequence from bovine specific mitochondrial DNA is amplified. The amplified specific sequence of the bovine mtDNA from meat and feed samples is demonstrated by both direct sequencing and restriction endonuclease digestion yield analysis. This method can detect bovine mtDNA in those less than 0.125% of bovine meat. Owing that this method is specific, rapid and sensitive, it can be utilized as a routine control assay in prevention of spreading of bovine spongiform encephalopathy in China caused by imported food and animal feeds especially meat and bone meals.