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白细胞介素-6相关新基因的生物学分析 被引量:1

Biological analysis of a novel gene related with interleukin-6
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摘要 目的通过白细胞介素-6(IL-6)相关新基因的生物学分析,研究IL-6的作用机制。方法利用IL-6相关表达序列标签(EST)进行电子克隆获得924 bp全长新基因,后采用RT-PCR方法从IL-6激活的人U937细胞所提的总RNA中钓取,此片断连到pGEM-Teasy质粒上并测序。结果成功钓取了IL-6相关EST电子克隆的全长cDNA基因。结论使用电子克隆技术对于发现新基因有重要的指导意义。 Objective To understand the action mechanism of interleukin (IL)-6 through investigation of its related genes. Methods Using electronic cloning with IL-6-related expressed sequence tags (ESTs), a novel gene at its full length of 924 bp was acquired. Reverse transcriptase-PCR method was subsequently employed to amplify the cDNA of this gene from the total RNA of human U937 cells, which were previously activated with 100 ng/ml IL-6 for 8 h. The fragment was then ligated into the pGEM-Teasy vector and sequence analysis was conducted. Result The whole cDNA gene was successfully fished. Conclusion Electronic cloning technology can be instrumental in identifying novel genes.
出处 《第一军医大学学报》 CSCD 北大核心 2003年第1期34-37,共4页 Journal of First Military Medical University
基金 国家杰出青年科学基金(39925019)~
关键词 白细胞介素-6 相关新基因 生物学分析 interleukin-6 U937 cells expressed sequence tags electronic cloning
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  • 1Shi Y,J Biol Chem,2000年,275卷,19167页
  • 2Xie M H,J Biol Chem,2000年,275卷,31335页

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  • 1Xia Q, Wang HX, Wang J. Proteomic analysis of interleukin 6-induced differentiation in mouse myeloid leukemia cells [ J]. Int J Biochem Cell Biol, 2005, 37(6): 1197-207.
  • 2Fischer P, Lehmann U, Sobota RM. The role of the inhibitors of interleukin-6 signal transduction SHP2 and SOCS3 for desensitization of interleukin-6 signalling [J]. Biochem J, 2004, 378(Pt 2): 449-60.
  • 3Hollestelle A, Schutte M. Representational difference analysis as a tool in the search for new tumor suppressor genes [J]. Methods Mol Med, 2004, 103: 143-60.
  • 4Ramalho-Ortigao JM, Temporal P, Oliveira SM. Characterization of constitutive and putative differentially expressed mRNAs by means of expressed sequence tags, differential display reverse transcriptase-PCR and randomly amplified polymorphic DNA-PCR from the sand fly vector Lutzomyia longipalpis [ J]. Mem Inst Oswaldo Cruz, 2001, 96(1): 105-11.
  • 5Tang G, Samaranayake LP, Yip HK. Direct detection of Actinomyces spp from infected root canals in a Chinese population: a study using PCR-based, oligonucleotide-DNA hybridization technique [J].J Dent, 2003, 31(8): 559-68.
  • 6Cross JT, Benton HP. The roles of interleukin-6 and interleukin-10in B cell hyperactivity in systemic lupus erythematosus[J]. Inflamm Res, 1999, 48(5): 255-61.
  • 7黄耀江,王关林,黎燕,施明,王建安,胡美茹,沈倍奋.用一个简单快速的RT-PCR技术筛选白细胞介素-6作用相关基因[J].生物化学与生物物理进展,2001,28(4):563-567. 被引量:3

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