抗磷脂抗体对巨噬细胞摄取氧化型低密度脂蛋白及CD36表达的影响

Effect of Antiphospholipid Antibody on Uptaking of Oxidized Low Density Lipoprotein and Expression of CD36 in U937 Cells

为了探讨抗磷脂抗体对巨噬细胞摄入氧化型低密度脂蛋白的影响及其主要清道夫受体CD36抗原表达的调节 ,将培养的U937细胞与氧化型低密度脂蛋白 (80mg L)和抗磷脂抗体 (10 0mg L)孵育 ,用酶荧光法观察人类单核细胞株U937内脂质的变化、逆转录—聚合酶链反应和流式细胞术观察CD36抗原mRNA和蛋白水平表达的改变。结果发现氧化型低密度脂蛋白存在时细胞内胆固醇和胆固醇酯含量分别为 2 4 3± 9mg g细胞和 2 89± 12mg g细胞 ,流式细胞仪测CD36抗原为 2 5 % ,凝胶电泳分析CD36mRNA β actionmRNA为 1.0 5 ;在加入抗磷脂抗体后 ,胞浆内胆固醇和胆固醇酯明显增加 ,分别为 2 76± 10mg g细胞和 4 78± 13mg g细胞 ,流式细胞仪测CD36抗原为 37% ,凝胶电泳分析CD36mRNA β actionmRNA为 1.90 ;两组相比差异有显著性 (P <0 .0 5 )。以上提示抗磷脂抗体有促进U937细胞摄取氧化型低密度脂蛋白的作用 。

Aim To investigate whether antiphospholipid antibody increases the uptaking of oxidized low density lipoprotein and up regulates the expression of CD36 in U937 cells. Methods The cultured U937 cells were incubated with oxidized low density lipoprotein of 80 mg/L and antiphospholipid antibody of 100 mg/L for 48 hours in vitro. Then the contents of cholesterol and cholesterol ester were detected by enzyme fluorescence. The expression of CD36 on U937 was quantified by reverse transcription polymerase chain reaction (RT PCR) and flow cytometry respectively. Results In the present of oxidized low density lipoprotein, the uptaking of cholesterol and CD36 expression increased significantly after by U937 cells incubated with antiphospholipid antibody. Conclusion Antiphosphlipid antibody may accelerate atherosclerosis by up regulating CD36 expression.