TNF-α的表达及sTNF-R水平与早期自然流产相关性的研究

The relation between the expression of tumor necrosis factor-α and soluble tumor necrosis factor receptor in early prognancy and the spontaneous abortion

目的 研究肿瘤坏死因子 (TNF α) ,可溶性肿瘤坏死因子受体 (sTNFR)Ⅰ和Ⅱ与早期自然流产的关系。方法 对孕 3mo内 2 0例正常妊娠、2 0例第 1次自然流产 (spontaneousabortion ,SA)和 15例反复自然流产 (re currentspontaneousabortion ,RSA)妇女的绒毛和蜕膜组织应用免疫组织化学SP法检测TNF α ,应用双抗体夹心ELISA法检测上述妇女血清中sTNFRⅠ与sTNFRⅡ。结果 ①SA及RSA绒毛细胞滋养层和蜕膜中TNF α的表达均明显高于对照组 ;合体滋养层中TNF α的表达SA与对照组无显著性差异 ,而RSA显著低于对照组 (P <0 .0 5 ) ;②SA与对照组比较 ,sTNFRⅠ差异有显著性 (P <0 .0 5 ) ,sTNFRⅠ与sTNFRⅡ在RSA中的水平较SA均有显著差异 (P <0 .0 1)。结论 TNF α ,sTNFR与自然流产的发生发展有关。因某些病理情况引发的蜕膜中TNF α表达增加可能是自然流产发生的一个原因 ,而sTNFR水平的升高则可能具有自我保护和自我稳定的生理意义。

Objective To investigate the relation between the expression of tumor necrosis factor α(TNF α) and soluble tumor necrosis factor receptor (sTNFR) in early pregnancy and spontaneous abortion. Methods Chorionic villi and decidua and serum of first trimester were obtained from 20 normal pregnancies, 20 the first time spontaneous abortion(SA) and 15 recurrent spontaneous abortion(RSA) cases. The expression of TNF α was detected using immunohistochemical staining (S P), sTNFR were measured by sandwich ELISA. Results ①)TNF α expression in the cytotrophoblasts and decidua of SA and RSA were signigicantly higher than that of normal early pregnancy (P<0.05). In the syncytiotrophoblasts, TNF α expression of normal early pregnancy was similar to that of SA and it's significantly higher than RSA. ②The seurm sTNFRⅠ,sTNFRⅡof SA were significantly higher than those in the control group (P<0.05), sTNFRⅠand sTNFRⅡin RSA were significantly higher than those in the SA (P<0.05). Conclusions TNF α, sTNFR are associated with the oncogenesis and development of spontaneous abortion. An elevated expression of TNF α may be a cause of spontaneous abortion and there are important practical values to the elevated expression of sTNFR.[