单核细胞趋化蛋白-1在异位内膜组织中的表达

Expression of monocyte chemotactic protein-1 in endometriotic tissues

目的 :探讨单核细胞趋化蛋白 1(MCP 1)在子宫内膜异位症发生发展中的作用。方法 :采用免疫组织化学链酶亲和素 生物素 过氧化酶复合物染色法 (SABC法 )及电子计算机图象分析技术 ,分析 2 0例子宫内膜异位症患者的异位内膜与在位内膜中MCP 1的表达 ,并以 2 0例正常子宫内膜作对照组。结果 :MCP 1分布在子宫内膜腺上皮和间质细胞的细胞质中 ,且在腺上皮中的表达高于间质 (P <0 .0 1) ,与月经周期无关。子宫内膜异位症异位内膜与在位内膜中MCP 1呈高表达状态 ,与正常内膜相比 ,差异有高度显著性 (P <0 .0 1)。而在位内膜与异位内膜之间差异亦有高度显著性 (P <0 .0 1)。结论 :MCP

Objective:To investigate the role of monocyte chemotactic protein-1 in the pathogenesis of endometriosis.Methods:Immunohistochemical SABC method and computerized image analysis technology were employed to detect the expression of MCP-1 in the ectopic endometrium and eutopic endometrium of 20 patients with endometriosis.20 normal endometrium served as controls.Results: MCP-1 was located in the cytoplasma of both epithelial glands and stroma.The MCP-1 immunostaining was stronger in epithelial glands than that in stroma regardless of the menstrual cycle phase(P<0.01).The expression level of MCP-1 in endometriotic tissues and eutopic endometrium of patients with endometriosis was higher than that in normal endometrium(both P< 0.01).There was also a significant difference between endometriotic tissues and eutopic endometrium(P<0.01). Conclusion:MCP-1 may play an important role in the pathogenesis of endometriosis.