摘要
将微透析(microdialysis)高效液相色谱法(highperformanceliquidchromatography,HPLC)联用技术应用于定量研究超氧化物歧化酶(superoxidedismutase)与组氨酸和组氨酸 -金属离子配合物的相互作用.实验结果表明:Cu2 Zn2 SOD酶不仅与组氨酸,而且还可以与组氨酸 Co(Ⅱ)和组氨酸 Ni(Ⅱ)相互作用,Cu2 Zn2 SOD酶中的部分Cu(Ⅱ)和Zn(Ⅱ)被诱导和置换出来,并相应地影响了酶的活性;组氨酸 -金属离子配合物的作用比组氨酸强,酶活性降低也更明显;酶中结合的外来金属离子的量比从酶上被诱导和顶替下来的金属离子的量要少。
In this paper, a method of microdialysis coupled with high performance liquid chromatography (HPLC was established to study the quantitative interaction of superoxide dismutase ( Cu2Zn2SOD) with histidine and histidine-metal ion complexes. It was observed that not only histidine, but also histidine-Co(II) and histidine-Ni (II) could induce and substitute some Cu(II) and Zn(II) from Cu2Zn2SOD, eventually influence the activity of this metalloenzyme. Histidine-metal ion complexes have stronger ability than histidine to displace the bound metal ion from SOD. In addition, the amount of foreign metal ion binding to the SOD was less than that of metal ion lost from SOD. It obviously means that there was apoenzyme in the solution.
出处
《化学学报》
SCIE
CAS
CSCD
北大核心
2003年第1期95-99,共5页
Acta Chimica Sinica
