人胃癌细胞RNA转染树突状细胞诱导特异性抗肿瘤免疫的实验研究

Human Dendritic Cells Transfected with RNA from Gastric Carcinoma Induce Specific Antitumor Immunity

目的 观察人胃癌细胞株RNA转染的树突状细胞 (DC)所诱导的特异性抗肿瘤免疫效应 ,探讨肿瘤细胞RNA直接转染DC进行胃癌生物治疗的可能性。方法 胃癌细胞株AGS体外培养 ,提取RNA ;正常人外周血 ,进行体外DC的培养扩增 ;胃癌RNA直接转染DC ,MTT法检测胃癌RNA活化DC所诱生的淋巴细胞对胃癌AGS、视网膜母细胞瘤SoRb - 70的体外杀伤效应。结果 正常人外周血来源的DC ,经胃癌AGS细胞株RNA直接转染后 ,成功诱导特异性抗肿瘤免疫反应 ,AGSRNA组CTL在靶效比为 2 0∶1时对AGS、SoRb - 70的杀伤率分别为 84.5 4%、1.5 3 % ,而对照组对这两种细胞的杀伤率分别为 1.3 4%和 1.70 %。结论 肿瘤RNA转染DC可以诱导特异性抗肿瘤免疫 ,是一种有前景的胃癌治疗手段 。

Objective To observe the specific anti-tumor immunity of dendritic cells (DC) transfected with RNA from human gastric carcinoma cell line, and to explore the possibility of utilizing tumor RNA-transfected dendritic cells as a biological treatment modality for gastric ceancer. Methods Dendritic cells were cultured and amplified from peripheral blood of healthy donors and then were directly transfected with total RNA extracted from human gastric cancer cell line AGS. Lymphocytes from the same donor were cocultured with the transfected DC for activation. In vitro killing effects of activated lymphocytes targeting AGS and SoRb-70 were measured by the MTT method. Results Dendritic cells transfected with RNA from human gastric cancer cell line AGS were able to induce specific anti-tumor immunity. In the experiment group, the killing rates of CTL for AGS and SoRb-70 were 84.54% and 1.53% respectively, at an effector: target ratio of 20:1, whereas in the control group the killing rates of CTL for the two cell lins were 1.34% and 1.70% respectively. Conclusion Tumor RNA-transfected dendritic cells are able to induce specific anti-tumor immunity and represent a promising approach for the treatment of gastric carcinoma and deserve further research.