^(60)Coγ射线诱导的K562细胞增殖抑制和凋亡的研究

^(60)Co Gamma Ray-induced K562 Cell Proliferation Inhibition and Apoptosis

目的 观察不同剂量的γ射线和照后不同时间的K5 62细胞的增殖抑制及凋亡情况。方法 细胞动态计数 ;3H -TdR掺入分析 ;利用ApoAlertMitochondrialMembraneSensorKit在荧光显微镜下观察荧光情况和在流式细胞仪上测定凋亡细胞的比例。结果 在不同剂量照射和照后的不同时间处 ,6 0 Coγ射线诱导的细胞增殖抑制和凋亡情况明显不同 :细胞增殖抑制情况与受照剂量的大小呈正相关 ,细胞凋亡比例与受照剂量的大小未见明显相关性 ,而表现为在照后 6h处凋亡细胞较多。结论 6 0 Coγ射线照射引起的肿瘤细胞增殖抑制有一定的发生时机 ,并且表现出剂量相关性 。

Objective To study the proliferation inhibition and apoptosis effects of 60Co gamma rays on K562 cell line at different doses and time after exposure. Methods Dynamic cell counting, 3H-TdR incorporation assay,observation of different fluorescence intensity in living cells and apoptotic cells under a fluorescence microscope and detection of the fraction of the apoptotic cells by FCM(flow cytometry) using a 'ApoAlert Mitochondrial Membrane Sensor Kit'. Results Different doses of gamma rays caused different proliferation inhibition and apoptosis;cell proliferation inhibition is positively related to the dose they received,but the apoptotic fraction isn′t.Instead,we found that after 5 Gy of gamma rays exposure,the apoptotic fraction at 6 h is higher than that the at other timespot observed. Conclusion 60Co gamma ray-induced tumor cell proliferation inhibition occurs at special time and is positively related to the dose.But the 60Co gamma ray-induced cell apoptosis observed does not show obvious correlation to dose,it is more related to time after gamma ray exposure.