长春瑞滨联合外照射对人乳腺癌MCF-7细胞作用的研究

Studies on Effects of Vinorelbine Plus Radiation on Human Breast Cancer Cell Line MCF-7

目的 研究长春瑞滨联合外照射对人乳腺癌MCF - 7细胞的抗肿瘤机制。方法 采用MTT法检测长春瑞滨对MCF - 7细胞毒作用 ,流式细胞仪检测各组细胞周期分布、细胞凋亡指数 ,电子显微镜观察MCF - 7细胞凋亡的形态学特征。结果 MTT检测表明 ,长春瑞滨给药 2 4h的IC50 为 7.2 0 μg/ml。流式细胞仪细胞周期分析显示 :C组细胞大多处于G1～S期 ,V组G2 相细胞明显高于对照组 ,V组G2 相细胞与C组及R组相比均差异显著 (均P <0 .0 5 )。流式细胞仪检测各组MCF - 7细胞凋亡结果显示 :R组、V组、V +R组在不同时间内的平均凋亡指数分别为 2 .98± 3 .90 %、8.0 1± 1.87%、15 .5 7± 5 .2 3 % ,(V +R)组与V组及R组相比均差异显著 (均P <0 .0 5 )。透射电镜观察到MCF - 7细胞凋亡的形态学表现为染色质边集 ,胞浆浓缩 ,核固缩 ,可见有膜包绕的凋亡小体脱落。结论 长春瑞滨对乳腺癌MCF - 7细胞具有显著的细胞毒作用。它可诱导乳腺癌MCF - 7细胞生长阻止在G2 期 ,并能诱导它的凋亡。在照射前使用 ,能增强放疗对MCF -

Objective To investigate the mechanism of the anticancer action of vinorelbine combinated with radiation on human breast cancer cell line MCF-7. Methods The MTT method was applied to examine the growth inhibition of MCF-7 treated with vinorelbine. The cell cycle distribution and apoptosis index(AI) were analysed by flow cytometry. The morphology of the MCF-7 cells apoptosis was observed by transmission electron microscopy. Results The MTT result showed the IC 50 of vinorelbine for 24 h was 7.20 μg/ml. Cell cycle analysis showed the four groups have the different cell cycle distribution. In control group, many cells were in the G 1～S phase; in vinorelbine group, the percentage of the cell population in G 2/M was significantly different from that of control group or radiation group (P<0.05).The apoptotic indexes(AI) of MCF-7 of four groups showed: The mean AI at different time of radiation group, vinorelbine group and vinorelbine plus radiation group were 2.98±3.90%,8.01±1.87%,15.57±5.23%, the AI of (V+R) group was significantly different from that in the vinorelbine group or radiation group (P<0.05).Transmission electronmicroscope can detect condensed chromatin, nuclear fragmentation and apoptotic body of MCF-7 cells.Conclusion Vinorelbine can inhibit the growth of human breast cancer cell line MCF-7. It can cause accumulation of MCF-7 cells in the G 2/M phase of the cell cycle, and can induce its apoptosis. Vinorelbine pretreatment before radiation can potentiate the effects of radiation and apoptosis induced by radiation.