低能量氦-氖激光对周围神经再生的运动神经传导速度研究

An motor nerve conduction study of low energy He-Ne laser on regeneration of peripheral nerve of rabbit

目的该研究通过对兔腓总神经传导速度等多项目的观察,探索低能量氦-氖激光对周围神经再生的影响。方法用44只体重2.5kg左右的家兔随机分为4,8,12及16周4个观察组,照射组各用兔6只,对照组各用5只。麻醉后,均切断左侧腓总神经,用9/0尼龙单丝对端吻合神经外膜。照射组在术后1天开始用8318-B型低能量氦-氖激光仪经皮肤照射L5～6脊髓节段,每天照射15min,共照射14d。对照组不照射,均按期观察。结果术后4周,可在照射组看到细小而稀少的再生轴突,对照组直到术后8周才能看到(P<0.01)。照射组的腓总神经运动神经传导速度均优于对照组(P<0.01),动作电位波幅照射组也优于对照组。胫前肌肌纤维横纹,照射组16周时很清楚,对照组不甚清楚。展趾功能到术后16周时,照射组与健侧相同,对照组才恢复到照射组12周的水平。结论低能量氦-氖激光促进了脊髓运动神经细胞功能,加速了轴突再生,促进神经修复后肢体功能的恢复。

Objective To elucidate the influence of the low energy He-Nelaser on the function of regeneration of peripheral nerve.Methods Forty-four rabbits about 2.5kg body weight were used in the experiment.T he animal were divided into 4,8,12,16weeks groups according to the observation period.Six animals were used in each irradiated group and in the control g roup 5rabbits were used in each observation period.The regeneration of the axon andmyelin sheath,the motor nerve conduction of the common p eroneal nerve,the conditions of the anterior tibital muscle and the t oe expansion test were all observed s ystematcally in the both group.Results A few thin regenerated axon was seen at 4weeks in the irraditated group,while in the control group it m ight be seen at 8weeks(P<0.001).A low amplitude motor nerve conduction of the common peroneal nerve was determined at the peroneal side of the anteriortibial muscle in a f ew animal at 4weeks of the irradiated group,and it was not obse rved in the control group,from 12to 16weeks.The motor nerve conduction o f the common peroneal nerve was the irradiated group than in the c ontrolled(P<0.01).The amplitude of the irradiated gro up was the higher than in the controll ed.The regeneration of the myelin sheath wa s evident in theirradiated group,an d also the section of the muscle fibers anterior tibial was clearly visibl e than the controlled.16weeks postop eratively,the toe expansion test wa s normal in the irradiated group,whi le in the control group it was the same asseen at 12weeks after operation in the irradiated group.Conclusion Now it was certain that the low energy He-Ne laser could promote the function of the spine motor nerve cells and accelerate the axo nal regeneration.