Expression of neuron-specific enolase and gial fibrilous acidic protein in cerebral tissue of experimental cerebral concussion

Objective To study the changes and significance of neuron-specific enolase(NSE)and glial fibrilous acidic protein(GFAP)in rat cerebral concussion.Methods80Wistar male rats were used for anim al model of cerebral concussion,whi ch were sacrificed on the 1st,3rd,7th,14th and 30th days after injury a nd the brain tissue were taken off.The expressions of NSE and GFAP were stu died in the course of cerebral concussion by means of immunohistochemistry.Results Rats in 100g-group were seen the clin ical manifestation for typical concussion.The pathologic changes were the cerebra l vascular constriction and dilatio n,congestion and edema of cerebral t issue and neuronal degeneration and necrosis.NSE was increased on the 1s t day,and the positive area was seen i n the plasma of the neurons in the cere bral cortex and the cerebellum,and also seen in blood vessels,cereb rospinal fluid in aqueduct and interstitial matrix.NSE was obtained at peak on the 7th day,decreased on the14th day and still raised on the 30th d ay.GFAP was increased on the 1st day,which the positive area was seen in th e plasma of astrocytes,and obtained at peak on the 3rd day,which fiber-like GFAP was in short,thick a nd astrocytes increased.GFAP decre ased on the 7th day and obtained normal level in 30days.Conclusion The main pathologic changes of cereb ral concussion were blood circulato ry disorder and nervous cells de-generation,apoptosis and necrosis.NSE and GFAP participated in the cou rse of cerebral concussion,may play an important role in the damage of blood-brain barrier,nervous cells degeneration and necrosis.