摘要
本试验制成大豆未成熟子叶原生质体再生细胞分裂率高、能保持胚性状态并最终能分化成正常植株的培养基。试验发现,天冬酰胺、谷氨酰胺、尿囊素和尿囊酸等大豆根瘤形成的酰尿有机态氮是有效的主要氮源。
The studies indicated that asparagine,glutamine,allantoin,and allantoic acid—ureide
organic nitrogen formed by soybean root nodules—were the major effective nitrogen sources
in successful soybean protoplast culture.Soybean regenerated plants were obtained in the
studies when the media containing organic nitrogen sources mentioned above were used.
关键词
大豆
原生质体
培养基
愈伤组织
soybean
protoplast
medium
embryogenic callus
allantoin
allantoic acid
plant regeneration
