P27和cyclinD1在慢性萎缩性胃炎癌变过程中的表达及意义

Expressions of P27 and cyclinD1 in the cancerous process from chronic atrophic gastritis and its significance

目的 探讨P2 7和cyclinD1在慢性萎缩性胃炎伴大肠化生转变为胃腺癌过程中的表达及意义。方法 采用免疫组织化学S—P法 ,检测正常胃粘膜、慢性萎缩性胃炎伴大肠化生和胃腺癌组织中P2 7和cyclinD1的表达。结果 在正常胃粘膜、慢性萎缩性胃炎伴大肠化生和胃腺癌中P2 7表达率分别为 75 .0 %、4.0 %、15 .0 % ,cyclinD1表达率分别为 2 0 .0 %、5 5 .0 %、75 .0 % ,正常胃粘膜中P2 7和cyclinD1的表达均较其它两类病变有显著差异 (P <0 .0 5 ,P <0 .0 1)。相关性分析显示 ,P2 7和cyclinD1在胃腺癌组织中呈反向表达。P2 7失活和cy clinD1过表达与胃腺癌浸润程度和有无淋巴结转移有关 (P <0 .0 5 ,P <0 .0 1)。结论 P2 7失活和cyclinD1过表达可作为慢性萎缩性胃炎早期癌变的分子指标 。

Objective To study the expressions and effects of P27 and cyclinD1 in the cancerous process from chronic atrophic gastritis accompanied by large-intestine-metaplasia to adenocarcinoma.Methods The expressions of P27 and cyclinD1 were determined in the specimens of normal gastric mucosa, chronic atrophic gastritis with large-intestine-metaplasia and gastric adenocarcinorma by immunohistochemistry(S-P).Results Positive immunostaining rate for P27 protein was the highest in normal gastric mucosa(75.0%,15/20) and decreased with progression from chronic atrophic gastritis to adenocarcinoma (40.0%,15.0% respectively).There were significant differences between chronic atrophic gastritis, gastric adenocarcinorma and normal gastric mucosa group(P<0.05,P<0.01 respectivel). Positive immuostaining of cyclinD1 from normal gastric mucosa (20.0%,4/20) to chronic atrophic gastritis (55.0%,11/20) to adenocarcinorma (75.0%,15/20) were increasing. There were dramatic differences between chronic atrophic gastritis, gastritis adenocarcinoma and normal gastric mucosa group(P<0.05,P<0.01). An interesting observation showed that inverse expression between P27 and cyclinD1 in most of the gastritis adenocarcinoma tested. The excessive expressions of cyclinD1 and inactivity of P27 were correlated with the invasive depth of adenocarcinoma and lymphaden metastasis.Conclusion These data have proved that P27 and cyclinD1 may be regarded as molecular marker of early carcinogenesis in chronic atrophic gastritis and be related to prognosis of gastric adenocarcinoma.