蛇床子素对家兔离体小肠平滑肌活动的影响

Effects Of Osthol on Activity of Isolated Small Intestinal Smooth Muscle form Rabbit

目的 :观察蛇床子素 (Ost)对家兔离体小肠平滑肌活动的影响。方法 :采用常规离体灌注小肠平滑肌标本运动的实验方法 ,记录用药前后肠段平滑肌自发收缩活动的特点及其变化规律。结果 :不同浓度 ( 1 μmol/L～ 5 0 μmol/L)的ost对小肠平滑肌自发收缩活动有抑制效应 ,其幅值变化与用药前比较有显著性差异 (P <0 .0 5 ,P <0 .0 1 ,P <0 .0 0 1 ) ,呈剂量依从性关系 ;较高浓度 ( 1 0 μmol/L ,5 0 μmol/L)Ost可显著减弱外源性乙酰胆碱和高Ca2 +诱导的小肠平滑肌收缩幅度的增强效应 (P <0 .0 0 1 )。结论

Objective:To investigate the effects of osthol(ost) on activity of isolated Rabbit small intestinal muscle.Methods:Using the routine experimental method of small intestinal smooth muscle isolated from Rabbit,we recorded the auto matic contracting characteristic and changing law in taking drugs before and after.Rusults:Ost of diffirent density(1μmol/L～50μmol/L)have a restraining effect on automatic contrating activity of isoluted small intestinal smooth muscle,whose scope have remarkable diffirence comparing to taking drugs before and after(P<0.05,P<0.01,P<0.001)and showed a dose-response dependent relationship.Thicker density (10μmol/L,50μmol/L)ost can restrain contrating by acetylcholine and thicker Ca 2+ (P<0.001).Conclusion:Ost can decrease convulsion of small intestine and resist effect of Ca 2+ .