中国对虾养成期细菌性黑鳃、褐斑综合症的病原生物学研究

Pathogenetic biology studies on black gill and brown spot of shell disease syndrome of penaeid shrimp infected by bacteria

本文是对中国对虾黑鳃、褐斑综合症进行病原菌分离和人工感染试验的报告。经细菌学鉴定表明,分离菌分别为海弧菌Vibrio pelagius Baumann和溶藻弧菌Vibrio alginolyticus sakazaki。人工感染试验显示,海弧菌是引起对虾黑鳃、褐斑综合症的主要病原菌,溶藻弧菌的合并感染导致对虾败血死亡。此外还应用电子显微镜对分离纯化的病原细菌进行了超微结构观察。

This paper is a report on both the pathogenic bacteria isolation and an artificial infection test of the epizootic black gill and brown spot of shell disease syndrome. Bacteriological identification shows that the isolated bacteria are Vibrio pelagius and Vibrio alginolyticus. The artificial infection test shows that V. pelagius is the major pathogen causing black gill and brown shell spot disease syndrome which was found among penaeid shrimp (Penaeus chinensis) , and that infection of V. alginolyticus together with V. pelagius results in septicemia and high mortality of penaeid shrimp. In addition, the ultrastructure of the isolated pathogenic bacteria was observed by TEM.