一例细菌对MDCK细胞污染的检测

Detection of a Bacterial from Contaminated MDCK Cell

MDCK细胞培养液(含双抗)表面有一层薄的油状膜,培养液不混浊。细菌学检测为一种球菌污染。对污染菌株进行药敏实验。使用MEM(含双抗)多次清洗细胞培养瓶及细胞表面,细胞培养液中改加菌株敏感的恩诺沙星。细菌学检测结果表明,加入恩诺沙星有效地控制和清除了细胞中污染的该株细菌。

There was a thin oil like membrane on the surface of MDCK cell culture, which was still clear Identification of bacterium showed that it was contaminated by a kind of coccus Antibiotics' sensitivities of the coccus were detected After washing the bottle of cell culture and the cell surface with MEM (contains two sorts of antibiotics) for several times, and adding enrofloxacin to the cell culture, the MDCK cell has been away from contamination