高原地区自身免疫病患者抗ENA抗体谱与体液免疫的比较

COMPARISON ANTI-ENA OF AUTOIMMUNE DISEASES WITH HUMORAL IMMUNE AT HIGH ALTITUDE

目的 抗ENA抗体谱的临床意义与其他免疫指标的比较。方法 对 1 0 3例自身免疫患者血清标本 ,采用免疫印迹法检测抗体 ,经免疫散射比浊法检测免疫球蛋白和补体。结果 抗ENA抗体在自身免疫病有较高的检出率 ;与正常对照组比较 ;抗ENA抗体阳性血清中IgG、IgM、IgA增设 ,而C3和总补体溶血性 (CH50 )水平下降 ;随着抗ENA抗体免疫印迹条带数的增多 ,IgG增设 ,而IgM、C3及CH50有明显的下降 ;C3、CH50与疾病活动性有关系。结论 抗ENA抗体阳性者存在体液免疫应答增加 。

Objective To comparison the clinical significance anti-ENA lineage of autoimmune diseases with other immune markers. Methods Immunoglobulin and complement of 103 cases autoimmune diseases were detected, western-blotting and immunonephelometry were tested. Results The high positive rate of anti-ENA antibody in autoimmune diseases. The quantity of IgG, IgM and IgA were increasd but C 3 and 50% complement,hemolysis were decreased. number of bands of anti-ENA antibody by Western-blotting and IgG were increased, but IgM. C 3 and CH 50 were decreased singnigicantly .There is an association between clinical' activity of diseases and C 3,CH 50. Conclusion Humoral immune activation was demonstrated in patients with positive ENA autoantibody and positively correlated with the number of bands of anti-ENA autoantibodies.