大鼠急性胰腺炎胰腺细胞凋亡与外周血浆TNF-α的关系

The relationship between apoptosis and TNF-α in acute pancreatitis rats

目的 :探讨急性胰腺炎 (AP)胰腺细胞凋亡与外周血浆肿瘤坏死因子α(TNF α)之间的关系。方法 :将 4 8只SD大鼠随机分为两组 :胰腺炎组 (n =2 4 )和假手术组 (n =2 4 )。胆胰管逆行加压注射 4 %牛磺胆酸钠诱导大鼠AP模型 ,术后 1h、3h、6h、12h抽血后分批处死 ,应用末端脱氧核苷酸转换酶 (TdT)介导的原位末端标记法检测胰腺细胞凋亡 ,用放射免疫法测定外周血浆TNF α的含量。结果 :胰腺炎组术后 1h凋亡细胞进行性增多 ,6h后减少 ,6h凋亡指数显著高于 3h、12h(3h ,P <0 .0 5 ;12h ,P <0 .0 1) ;TNF α浓度 ,3h、6h较 1h明显降低 (3h ,P <0 .0 1;6h ,P <0 .0 1) ,12h较 6h明显升高 (P <0 .0 1)。结论 :诱导胰腺细胞凋亡是TNF α参与AP发病机制的一个重要因素 ,这种作用与TNF α自身的浓度有关。

Objective:To investigate the correlation between pancreatic acina cell apoptosis and plasm TNF α in acute pancreatitis(AP) rats.Methods:48 SD rats were divided randomly into two groups:acute pancreatitis(AP) and shan operation(SO). The model of AP was induced by retrograde injection of 4% sodium taurocholate into biliopancreatic duct in rats. At 1 h,3 h,6 h and 12 h respectively after operation,the blood sample was taken for the measerurement of plasm TNF α with ridoimminology,a terminal deoxynucleotidyl transferase mediated dUTP biotion nick end labeling(TUNEL) was used to detect apoptosis of pancreatic acinar cell.Results:Apoptosis index(AI) of AP rats at 6 h was significantly higher than that at 3 h,12 h(3 h,P<0.05;12 h,P<0.01,respectively),the level of plasm TNF α at 3 h?6 h was significantly higher than that at 1 h(3 h,P<0.05;6 h,P<0.01,respectively),while the level at 12 h was higher than that at 6 h(P<0.01).Conclusion:Pancreatic acinar cell undergoing apoptosis has a close relation to the level of TNF α in AP.