新生鼠出血肺组织中内皮素、降钙素基因相关肽与氧自由基的关系

The relationship between endothelin-1(ET-1), calcitonin gene related peptide(CGRP) and oxygenic free radical (OFR) in pulmonary tissue of newborn rats

目的 探讨出血肺组织中内皮素 -1(ET -1)、降钙素基因相关肽 (CGRP)与氧自由基 (OFR)的关系。方法 将 40只新生Wistar大鼠分成两组 ,正常对照组 10只 (A组 ) ,研究组 3 0只 (B组 ) ,用低温缺氧后复温供氧方法 ,制作肺出血模型 ,将肺组织制成匀浆 ,用放射免疫法检测ET -1及CGRP ,紫外分光光度计检测丙二醛 (MDA)及超氧化物歧化酶 (SOD)。结果 研究组 3 0只新生鼠均发生肺出血 ,肺出血由轻至重共 3种类型 ,包括点状出血 8只 ,局灶出血 12只 ,弥漫出血 10只。随着肺出血加重 :①肺组织ET -1及CGRP含量不断升高 (与对照组比较P <0 0 0 1) ,3种类型出血中 ,ET -1组间差异有显著性 (P <0 0 5～ 0 0 0 1) ,而CGRP则组间无差异 (P >0 0 5 )。②肺组织MDA不断升高 ,SOD不断下降 ,各组间差异有显著性 (P <0 0 5～ 0 0 0 1)。结论 ①机体对低温缺氧后复温供氧耐受力的不同导致肺组织ET -1及CGRP释放量亦不同 ;②可导致不同程度肺出血的OFR ,乃由ET

Objective To investigate the relationship between ET-1, CGRP and OFR in pulmonary tissue of newborn rats. Methods Pulmonary hemorrhage (PH) model was established in 40 newborn Wistar rats which were divided into 2 groups. Group A (10 rats) was normal control and group B (30 rats) was in a situation of low temperature and hypoxia followed by normal temperature and high oxygen. ET-1 and CGRP, molondaildehyde(MDA) and superoxide dismutase (SOD) were detected by radioimmunossay and spectrophotometer in the lung tissue, respectively. Results There were 3 categories from mild to severe hemarrhage including spotty PH(8 rats), local PH(12 rats) and diffuse PH(10 rats ) in group B. As the severity of PH increased:①ET-1 and CGRP increased progressively (compared with group A, P <0 001), but ET-1 and CGRP had not significant difference between 3 categories pH, respectively( P <0 05～0.001 and P >0 05). ②MDA increased and SOD decreased progressively with significant difference( P <0 05～0 001). Conclusion ①Different tolerance to low temperature, hypoxia followed by normal temperature and high oxygen, the ET-1 and CGRP in lung tissue has different release. ②The OFR resulting in PH of different degree is crased by ET-1 through the autocrine action on pulmonary vascular endothelial cell.