乙型肝炎患者血清HBV DNA与HBV-M检测比较分析

Comparatine analysis of HBV DNA and HBV-M in the serum of patients with hepatitis B

目的 :探讨不同免疫标志物的乙型肝炎 (乙肝 )患者血清HBVDNA阳性率及其临床意义。方法 :用PCR方法检测乙肝患者血清HBVDNA ,用ELISA方法检测乙肝五项标志物即HBsAg、HBsAb、HBeAg、HBeAb、HBcAb。 结果 :2 12例HBsAg、HBeAg、HBcAb均阳性的标本HBVDNA也全部阳性 ,阳性率为 10 0 % ;15 8例HBsAg、HBeAb、HBcAb均阳性的标本 ,HBVDNA 6 2例阳性 ,阳性率为 39.2 % ;HBsAg、HBcAb阳性标本 5 5例 ,其中 30例HBVDNA阳性 ,阳性率为 5 4 .5 % ;三抗体HBsAb、HBeAb、HBcAb阳性标本 4 0例 ,其中 5例HBVDNA阳性 ,阳性率为 12 .5 %。结论 :PCR方法更灵敏。

Objective:To explore the positive rate of HBV DNA in the serum of patients with hepatitis B in different markers and its clinical significance.Methods:Serum HBV DNA was tested by polymerase chain reaction(PCR),hepatits B virus markers(HBsAg?HBsAb?HBeAg?HBeAb?HBcAb) were detected using enzyme linked immunosorbent assay(ELISA).Results:In 212 HBsAg+/HBeAg+/HBcAb+ samples,all the PCR results were positive,the positive rate was 100%;In 158 HBsAg+/HBeAb+/HBcAb+ samples,HBV DNA positive rate was 39.2%(62/158);In 55 HBsAg+/HBcAb+ samples,HBV DNA positive rate was 54.5 %(30/55);In 40 HBsAb+/HBeAb+/HBcAb+ samples,HBV DNA positive rate was 12.5%(5/40).Conclusions:The results reveal that the PCR assay is more sensitive.Only by testing HBV DNA,can we monitor the true state of HBV infection and amplification.