摘要
目的研究脑缺血后大鼠海马CAl区神经元持续钠电流的变化,探讨钠通道阻滞剂对缺血脑组织保护的机理.方法酶消化法急性分离SD大鼠(10～14d)海马CA1区神经元,全细胞膜片钳技术记录脑缺血对持续钠电流的影响.结果缺血3min时持续钠电流增加到正常时的1.59+0.26倍(P＜0.05),缺血5 min时增加到正常时的2.92±0.46倍(P＜005).结论脑缺血时钠通道开放,持续钠电流增加.
Objective To study the variations of persistent sodium current of CA1 hippocampal neurons following cerebral ischemia in rats. Methods Whole-cell patch-Clamp recordings were made from enzymatically isolated rat CA1 hippocampal neurons. Results3 min and 5 min after the cerebral ischemia occurs, the persistent sodium current increases to 1.59±0.26 times and 2.92±0.46 times respectively compared to the normal persistent sodium current (P<0.05) . Conclusion Persistent sodium current of rat CA1 hippocampal neurons increases on condition of cerebral ischemia.
出处
《中华神经医学杂志》
CAS
CSCD
2003年第1期61-63,共3页
Chinese Journal of Neuromedicine
关键词
脑缺血
持续钠电流
钠通道
海马
膜片钳
cerebral ischemia
persistent sodium current
sodium channel
hippocampus
patch-clamp
