不同pH和温度条件下杂交鲟胃中消化酶活性的变化

Effects of pH and temperature on digestive enzyme activities in stomach of hybrid sturgeon (Huso huso♀×A. ruthenus♂)

以杂交鲟(Husohuso♀×A ruthenus♂)为研究材料,测定不同pH及不同温度条件下胃中蛋白酶、淀粉酶和脂肪酶的活性。结果表明,当pH2 2～7 8时,蛋白酶随pH的升高酶活性逐渐降低,淀粉酶及脂肪酶在偏碱性条件下才具有一定的酶活性,而且活性较弱。在20～40℃范围内,蛋白酶活性随温度的升高而增加,淀粉酶、脂肪酶分别在35℃和25℃处达最大酶活性。

Six hybrid sturgeons(Huso huso♀×A ruthenus♂)at 1+ year of age and body weight 510625 g were employed to test the activities of protease, amylase and lipase of stomach in vitro When pH was at the range of 2278 and water temperature at 30 ℃ the protease activity decreased gradually with pHincreasing, which showed protease has high activity only at low pH Amylase and lipase did not show any enzyme activities until pH increased to over 62, and their activities were lower than that of protease When water temperature was at the range of 2040 ℃ the protease activity increased with the temperature increasing and the activities of amylase and lipase got to their peaks at 35 ℃ and 25 ℃, respectively