脑醒喷鼻剂对大鼠脑缺血再灌注损伤脑组织超微结构的影响

Effects of Naoxing Nasal Spray on Ult rastructure of Brain Tissue in Rats with Cerebral Ischemia -reperfusion Injury

目的研究脑醒喷鼻剂对大鼠局灶性脑缺血再灌注损伤脑组织超微结构的影响。方法将30只Wistar大鼠随机分为脑醒喷鼻剂高剂量组(高剂组)、脑醒喷鼻剂中剂量组(中剂组)、脑醒喷鼻剂低剂量组(低剂组)、尼莫通腹腔注射组(对照组)、生理盐水喷鼻剂组(NS组)、假手术组(正常组),给药3d后,用线栓法阻断大鼠左侧大脑中动脉建立左大脑中动脉栓塞(L-MCAO)模型,缺血1h再灌注24h后,取左额叶皮层脑组织作电镜观察。结果脑醒喷鼻剂高、中剂组的神经细胞、胶质细胞及毛细血管等超微结构的损伤程度明显低于NS组,其中以高剂组损伤最轻,与尼莫通对照组无明显差别。结论从组织形态学角度证实,脑醒喷鼻剂能减轻脑缺血大鼠大脑皮层神经组织的损伤,对脑组织的缺血再灌注损伤有明显的保护作用。

Objective To study the effects of Naoxing Nasal Spray(NNS)on ultrastructure of brain tissues i n rats with focal cerebral ischemia -reperfusion inj ury(CIRI ).Methods Thirty Wistar rats were randomly all ocated to 6groups:Group A(treated with high dose of NNS ),Group B(treated with moderate dose of NNS),Group C(treated with low dose of NNS ),Group D(positive control group),Group E (treated with normal saline)and Group F (normal group).After medication for 3days,rat models of CIRI were established by the occlusion of left mid dle cerebral artery.Ultrastructur e of left fore-head cortical tissue was observed un der electron microscope after one -h our ischemia and 24-hour reperfusio n.Results The injury of neurons,gliocytes and capillaries in Group A and Group B was obviously milder than that in Group E and that in Group A was the mildest,the differences being not significant as compared with that in Group D.Conclusion NNS can reduce the ischemic damage of cerebr al cortex and obviously protect cere bral tissues in rats with CIRI.