复方仙灵脾注射液对大鼠局灶性脑缺血的实验研究

Experimental Study of Compound Epim edium Injection for Focal Cerebral I schemia in Rats

目的探讨复方仙灵脾注射液对大鼠局灶性脑缺血的治疗作用。方法将SD大鼠60只,随机均分为假手术组、模型组、川芎嗪对照组、复方仙灵脾大、中、小剂量组。通过结扎大鼠一侧大脑中动脉(MCAO)造成局灶性脑缺血模型,观察复方仙灵脾注射液对血浆中的血栓素、前列环素、血小板聚集、超氧化物歧化酶、丙二醛及脑梗塞面积的影响。结果复方仙灵脾注射液不仅能明显提高血浆中的前列环素水平,降低血小板聚集率,增加超氧化物歧化酶活力,而且能明显减少血栓素水平和丙二醛的含量,缩小脑梗塞面积。结论复方仙灵脾注射液在改善血浆TXA2-PGI2的平衡,抑制血小板聚集,增加脑血流量的同时,又能清除自由基,改善脑功能,减轻急性脑梗塞所致的缺血损伤。

Objective To observe the effect of compound Epi medium injection(CEI )on focal cerebral ischemia in rats.Methods Sixty Sprague -Dawsley rats were ran domly allocated to six groups:mimic operation group(n=10),model group(n=10),tetramethylpyrazine injection gro up(n=10),CEI groups(treated with large -,moderate -and small -dose of CEI respectively).Focal cerebral ischemia in rats was induced by occlusion of right middle cerebral artery and the effects of CEI on plasm a levels of TXA 2 and PGI 2 ,platelet aggregation ,SOD and MDA a ctivities and infarct size of brain were observed.Results CEI could increase PGI 2 level in plasma,lower platelet aggregation rate,increase SOD activity,decrease TXA 2 level and MDA content and lessen the i nfarct size of brain.Conclusion CEI can keep the balance of TXA 2 -PGI 2 ,inhibit platelet aggregation and i ncrease the volume of cerebral blood flow.Meanwhile,it can also elimi-nate oxygen free radicals,improve cerebral function and relie ve ischemic damage induced by acute c erebral infarction.