摘要
目的 探讨白细胞介素 13(IL 13)mRNA在哮喘儿童中表达的意义。方法 用逆转录 聚合酶链反应 (RT PCR)半定量法检测了外周血单个核细胞 (PBMC)中IL 13mRNA表达的改变。结果 急性期IL 13mRNA表达水平增高 ,激素治疗半年后的恢复期IL 13mRNA表达降低 ;病情越重 ,IL 13mRNA表达水平越高。结论 IL 13是导致气道慢性炎症的各种因素之一 。
Objective To study the role of IL 13 mRNA in children with asthma. Methods Using the technique of semi quantitative RT PCR, we measured the IL 13 mRNA (relative to β actin) in peripheral blood mononucleic cell (PBMC) in children with asthma before and after a six month course of inhaled glucocorticoids (GC) therapy. Results There was an increased levels of IL 13 mRAN in asthmatic children compared with normal controls; higher expression of IL 13 mRNA was observed in severe case. Conclusion IL 13 may be one of many factors resulting in bronchial chronic inflammation, the clinical effects of GC are at least partially mediated through the modulation of cytokine production.
出处
《上海医学检验杂志》
北大核心
2003年第1期28-30,共3页
Shanghai Journal of Medical Laboratory Sciences
基金
浙江省医药卫生科学研究基金资助项目 (9812 )
