黑曲霉VVTP84生产果糖转移酶

Preparation of fructosyltransferase from Aspergillus niger VVTP84

从产果糖转移酶的 11株菌株中筛选出一株黑曲霉VVTP84菌 .该菌株在含蔗糖的培养基中 ,最佳摇瓶发酵时间为 30h ,pH值为 7.0 ;当蔗糖质量浓度在 2 5 0g/L以内时 ,产酶与蔗糖质量浓度呈正相关 ;MgSO4 ·7H2 O和KH2 PO4 的添加量分别以控制在 1.5 g/L和 1.0

Aspergillus niger VVTP84 was selected from 11 strains for fructosyltransferase production. The (shaking flask) conditions of preparing fructosyltransferase were investigated. The results indicated that the optimum fermentation time and initial medium pH were 30 h and 7.0, respectively. It was also found that the enzyme activity was directly related to the sucrose concentration when it was below 250 g/L. And the maximum value of enzyme activity was obtained when the concentrations of MgSO 4·7H 2O and KH 2PO 4 were at 1 g/L and 1.5 g/L respectively.