微生物法制备抗抑郁药R-托莫西汀中间体

Preparation of Chiral Pharmaceutical Intermediate--R-3-chloro-1-phenylpropanol with Microorganisms

从 2 0株酵母菌中筛选出了两种还原 3 氯 1 苯丙酮生成高光学纯度R 3 氯 1 苯丙醇的较好菌株SaccharomycesB5和Candida 10 4 ,R 3 氯 1 苯丙醇的对映体过剩值为 10 0 % .研究了温度、pH、反应时间、底物质量浓度、菌体添加量等微生物培养条件及反应条件对两种菌种还原 3 氯 1 苯丙酮的影响 .以体积分数 5 %的乙醇为共底物 ,可使SaccharomycesB5和Candida 10 4转化底物的产率分别从 17%和 9%提高到 5 0 %和 4 8%

Saccharomyces B5 and Candida 104 which have activity of 3 chloropropiophenone reduction to R 3 chloro 1 phenylpropanol were isolated. The effect of co substrate, reduction time, pH, temperature, substrate concentration and cell dry weight on the bioreduction was studied.