乙型肝炎病毒感染的孕妇胎盘组织中CD3、CD68及IL-2的检测

Detection of CD3,CD68 and IL-2 in placentas of women infected with hepatitis B virus during pregnancy

目的 :通过检测受到及未受到乙型肝炎病毒感染的胎盘组织中的CD3、CD6 8及IL 2 ,并对二者加以比较探讨受HBV感染的孕妇胎盘组织中细胞介导的免疫应答反应情况 .方法 :对象分为 :① 30名乙肝病毒血清标志物阳性且胎盘亦受感染的孕妇 ,② 11名乙肝病毒血清标志物阳性但胎盘未受感染的孕妇 ,③ 7名乙肝病毒血清标志物阴性胎盘也未受感染的孕妇 .收集其胎盘组织标本 ,用免疫组织化学法分别检测胎盘组织中多种免疫细胞 (每张切片随机选取 10个放大 2 0 0倍视野作细胞计数 ,然后取平均值 )以及细胞因子 .结果 :各组CD3阳性的T淋巴细胞和CD6 8阳性的巨噬细胞 (Hofbauer细胞 )的相应平均计数分别为 :第 1组 3 6和 2 0 5 ,第 2组 1 0和12 2 ,第 3组 0 7和 10 6 .方差分析结果显示第 1组两项指标与第 2、3组比较有显著差异 (P <0 0 1) ,而 2、3组之间无显著差异 (P >0 0 5 ) .第 1组感染HBV的胎盘中的IL 2阳性率为77 8% ,而未感染HBV的第 2组胎盘中阳性率为 12 5 % ,第 3组阳性率则为 0 .可以看出HBV感染的胎盘中CD3阳性的T淋巴细胞和CD6 8阳性的巨噬细胞数显著增加 ,同时白介素 2(IL 2 )表达量也增加 .结论 :胎盘受到HBV感染时 ,T淋巴细胞和Hofbauer细胞介导的细胞免疫可能有所增强 .

AIM: To investigate the cellular i mm une response (CMI) in placentas from women who seroconverted to HBV before or du ring their pregnancies and to compare it with that of the controls by detecting CD3, CD68 and IL 2 in placentas. METHODS: Immunohistochem ical techniques were used to detect the various immune cells and the inflammator y cytokine present in placental tissue sections in the following three groups: G roup 1 of placentas from 30 women whose pregnancies were complicated by HBV infe ction in their placentas, Group 2 of placentas from 11 women whose pregnancies w ere complicated by HBV infection but not in their placentas and Group 3 of place ntas from seven women whose pregnancies ended in either term delivery or electiv e abortion without HBV infection in their placentas. RESULTS: The results of the study revealed a statistically significant increase in t he number of CD3 positive T cells and CD68 positive macrophages (Hofbauer cells) present in the placentas from Group 1 compared with that from Group 2 or Group 3 (4 and 20 5 versus 1 and 12 2, 0 7 and 10 6, respectively) (P< 0 05). In addition, the inflammatory cytokine interleukin 2 was detect ed in 77.8% of the placentas in Group 1 but was absent from all the placentas ev aluated from Groups 3. CONCLUSION: These findings demonstr ate an inflammation-mediated cellular immune response in the placentas from wome n whose pregnancies are complicated with HBV infection in their placentas .