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胎鼠表皮干细胞的分离培养及毛囊再生 被引量：2

In vitro culture of murine fetal epidermal stem cells and its relationship with the regeneration of follicle
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摘要 AIM: To culture fetal murine epidermal stem cells and follicular papilla cells in vitro and to investigate the regeneration of epidermis and follicle on animal models. METHODS: The fetal murine epidermal stem cells (ESCs) were isolated by adhering to collagen type IV and cultured in conditional medium in vitro. Then, the ESCs were identified by intergrin β 1 and keratin15 antibodies, and the cell cycle profile and colony forming efficiency (CFE) were also studied. Meanwhile the follicular papilla cells were cultured from single follicle and grown in fibrin gel. Finally, the ESCs and papilla cells were transplanted on the full skin wound of BALB/C nude mouse together and the regeneration of epidermis and follicle would be investigated in the following 8～10 weeks. RESULTS: The ESCs isolated by collagen type IV were intergrin β 1 and kerain15 staining positive. The cell cycle profile analysis showed that 94.9% of the ESCs were at G1 stage and only 3.5% at S stage. As the control group, 74.1% of keratinocytes were at G1 stage and 17.5% at S stage. The CFE of ESCs was 15.3% and that of keratinocytes was 6.7%. After covered by ESCs and papilla cells for 8～10 weeks, the regeneration of epidermis and follicle were observed on the back of the mouse. CONCLUSION: The epidermal stem cells and follicular papilla cells could be isolated and cultured in vitro, and the epidermal stem cells could take part in forming the new follicle and epidermis structure induced by follicular papilla cells. AIM: To culture fetal murine epidermal stem cells and follicular papilla cells in vitro and to investigate the regeneration of epidermis and follicle on animal models. METHODS: The fetal murine epidermal stem cells (ESCs) were isolated by adhering to collagen type IV and cultured in conditional medium in vitro. Then, the ESCs were identified by intergrin β 1 and keratin15 antibodies, and the cell cycle profile and colony forming efficiency (CFE) were also studied. Meanwhile the follicular papilla cells were cultured from single follicle and grown in fibrin gel. Finally, the ESCs and papilla cells were transplanted on the full skin wound of BALB/C nude mouse together and the regeneration of epidermis and follicle would be investigated in the following 8～10 weeks. RESULTS: The ESCs isolated by collagen type IV were intergrin β 1 and kerain15 staining positive. The cell cycle profile analysis showed that 94.9% of the ESCs were at G1 stage and only 3.5% at S stage. As the control group, 74.1% of keratinocytes were at G1 stage and 17.5% at S stage. The CFE of ESCs was 15.3% and that of keratinocytes was 6.7%. After covered by ESCs and papilla cells for 8～10 weeks, the regeneration of epidermis and follicle were observed on the back of the mouse. CONCLUSION: The epidermal stem cells and follicular papilla cells could be isolated and cultured in vitro, and the epidermal stem cells could take part in forming the new follicle and epidermis structure induced by follicular papilla cells.
作者 韩军涛 陈璧
机构地区 第四军医大学西京医院烧伤科
出处 《第四军医大学学报》 北大核心 2003年第1期90-91,共2页 Journal of the Fourth Military Medical University
关键词 胎鼠 表皮干细胞 毛囊乳头层细胞 组织工程
分类号 R318.5 [医药卫生—生物医学工程]
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参考文献6

  • 1[1]Talor G, Lehrer MS, Jensen PJ, Sun TT, Lavker RM. Involvement of follicular stem cells in forming not only the follicle but also the epidermis [J]. Cell, 2000; 102: 451-461.
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同被引文献12

  • 1Jahoda CA, Oliver RF, Reynolds A J, et al. Trans-species hair growth induction by human hair follicle dermal papillae [J]. Exp Dermatol, 2001 ;10(4): 229-237.
  • 2Elliott K, Stephenson TJ, Messenger AG. Differences in hair follicle dermal papilla volume are due to extracellular matrix volume and cell number: Implications for the control of hair follicle size and androgen responses [J]. J Invest Dermatol, 1999; 113(6):873-877.
  • 3Peus D, Pittelkow MR. Growth factors in hair organ development and the hair growth cycle [J]. Dermatol Clin, 1996; 14 (4):559-572.
  • 4Brigitte AR, Margarete SM, Ulrike BP, et al. Cultured dermal papilla cells of the rat vibrissa follicle. Proliferative activity, adhesion properties and reorganization of the extracellular matrix in vitro [J].Arch Dermatol Res, 1997; 289 (12):698-704.
  • 5Inamatsu M, Matsuzaki T, Iwanari H, et al. Establishment of rat dermal papilla cell lines that sustain the potency to induce hair follicles from afollicular skin [J]. J Invest Dermatol, 1998; 111 (5):767-775.
  • 6Cotsarelis G,Kaur P,Dhouailly D,et al.J.Epithelial stem cells in the skin:Definition,markers.localization and functions[J].Exp Dermatol,1999,8(1):80-88.
  • 7Tumbar T,Guasch G,Greco V,et al.Defining the epithelial stem cell niche in skin[J].Science,2004,303:359-363.
  • 8Mati M,Evans WH,Brink PR,et al.Epidermal stem cells do not communicate theough gap junctions[J].J Invest Dermstol,2002,118:110-116.
  • 9Akivama M,Smith,LT,Shinuzu H.Changing pottems of localisation ofputative stem cells in developing human hair folleles[J].J Invest Dermatol.2000,114(2):321-327.
  • 10Lavker RM,Miller SJ,Sun TT.Epithelial stem cells,hair follictes,and tumor formation[J].Recent Results Cancer Res,1993,128:31-41.

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第四军医大学学报
2003年 第1期

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