31例急性感染性多发性神经根炎患儿血清-脑脊液中抗GM_1抗体检测分析

ANALYSIS OF ANTIBODES DETECTION of IgG.IgM TO GAONLIOSIDE GM_1 IN THE SERUM AND CEREBROSPINAL FLUID OF 31 GUILLAIN-BARRE SYNDROMS

目的 检测急性感染性多发性神经根炎患儿急性期、恢复期血清及脑脊液中抗神经节苷脂GM1 IgG、IgM抗体变化 ,探讨该病的发病机制。方法 采用酶联免疫吸附试验方法研究 31例GBS患儿急性期、恢复期血清及脑脊液中抗神经节苷脂GM1 IgG、IgM抗体变化 ,设立两组对照组 ,即非GBS神经系统疾病组和正常组。结果 GBS患儿血清抗神经节苷脂GM1 IgM抗体OD均值高于二组对照组 (P <0 .0 5) ,脑脊液中抗GM1 IgM抗体OD均值高 ,与疾病对照组比较 (P <0 .0 5) ,血清抗GM1 IgG抗体OD均值高于正常组(P <0 .0 5)。结论 部分GBS患儿抗GM1 IgG、IgM抗体明显升高 ,尤其是在GBS亚型急性运动轴索神经病 (Acutemotoraxonalneur pathyAMAN)中升高显著 ,GM1 IgM抗体阳性率 75 %。表明 :神经节苷脂的免疫损伤在GBS发病中起重要作用 。

Objective To detect the serum antibodies IgG.IgM to ganglioside GM 1 of 31 patients with GBS, and explore its pathogenesis. Methods we set up two control grouPs, no GBS neurological diseases and the normal subjects.Detected the serum antibodies IgG.IgM to ganglioside GM 1 by enzyme-linked immunosorbent assay of 31 acute-phases and recovery patients with GBS. Results The serum level of anti-GM 1 IgG,IgM with GBS was significant higher than the other control groups(P<0.05).The cerebrospinal level was significamt higher than that normal group(P<0.05). Conclusion ganglioside GM 1 immunity damage is an important factor in GBS, especially in AMAN.