低氧对复合培养的平滑肌细胞中白细胞介素-6表达的影响

Enhancement of interleukin-6 expression in cocultured pulmonary arterial smooth muscle cells in hypoxia

目的 研究低氧对复合培养的平滑肌细胞中白细胞介素 6(IL 6)表达的影响。方法 低氧处理与肺微血管内皮细胞复合培养的鼠肺动脉平滑肌细胞 ,采用RT PCR方法检测常氧组、低氧 2、6、12h组IL 6基因的表达水平 ,并测定细胞培养上清中IL 6的活性。结果 低氧复合培养 2h组肺动脉平滑肌细胞中IL 6mRNA表达水平升高 ,在低氧 6h组达最高 ,低氧 12h组有所降低 ,但与常氧组比较有明显差别。低氧复合培养 2h组肺动脉平滑肌细胞上清中IL 6的活性明显升高 ,6h组细胞上清中IL 6的活性水平最高 ,12h组的活性降低 ,2 4h组的水平低于 2h组。IL 6活性水平与其基因表达的水平一致。结论 低氧可以增强复合培养的肺动脉平滑肌细胞中IL 6基因的表达 ,使细胞培养上清中IL 6活性升高 。

Objective To study the effect of hypoxia on the expression of interleukin 6 (IL 6) in cocultured pulmonary arterial smooth muscle cells (PASMC) Methods Rat PASMC were cocultured with rat lung microvascular endothelial cells, and randomly divided into 5 groups: normal group (N), 2 h hypoxia (H2), 6 h hypoxia (H6), 12 h hypoxia (H12), and 24 h hypoxia (H24) The expression level of IL 6 mRNA in PASMC was detected with RT PCR, and the activity of IL 6 in the supernatant with radioimmunoassay Results The expression level of IL 6 mRNA increased in PASMC in H2, reached the highest in H6, and decreased in H12, but still higher than that of N The changes of the IL 6 activity in the supernatant as well as IL 6 mRNA expression in the cells were in a time dependent manner Conclusion Hypoxia can enhance the expression of IL 6 in cocultured PASMC And it may activate others genes which regulate the hypoxic response of PASMC and signal transduction