MAPK在慢性髓细胞白血病K562细胞株信号转导中的作用

Effects of Mitogen Activated Protein Kinase(MAPK) in Cell Signal Transduction of Chronic Myelogenous Leukemia Cell Line K562

背景与目的:近年研究表明,ras-MAPK信号传导通路在慢性髓细胞白血病(CML)的发生和发展中起着重要作用。本研究拟探讨丝裂原活化的蛋白激酶(mitogen-activatedproteinkinase,MAPK)在CML信号传导中的作用及其作用机理。方法:用脂质体转染法将MAPK的反义寡核苷酸(antisenseoligodeoxynucleotide,ASO)导入CML细胞株K562细胞中,通过细胞增殖、DNA合成、MAPK含量和MAPK活性变化检测MAPKASO对K562细胞的作用。结果:MAPKASO可明显抑制细胞增殖、DNA合成、MAPK含量和MAPK活性,其抑制率分别为51.8%、57.1%、45.3%和61.6%,与对照组比较差异有统计学意义(P<0.05)。结论:MAPK在CML信号转导中起重要,极有可能成为治疗CML的新靶点。

BACKGROUND & OBJECTIVE:Ras MAPK signal transduction has been thought to play an important role in the carcinogenesis of chronic myelogenous leukemia. In this study, the authors investigated the effects and mechanism of mitogen activated protein kinase (MAPK) in cell signal transduction of chronic myelogenous leukemia(CML). METHODS:After MAPK antisense oligodeoxynucleotide (ASO) was introduced into K562 cell line by liposomal transfection, the effects of ASO on K562 cell were evaluated by cell proliferation,DNA synthesis,MAPK protein content and MAPK activity. RESULTS:The cell proliferation, DNA synthesis, MAPK protein content and MAPK activity were significantly inhibited by MAPK ASO, the inhibitory rates were 51 8%, 57 1%, 45 3%, and 61 6%, respectively,with significant difference in comparison to the control (P< 0 05). CONCLUSION:MAPK plays an important role in the signal transduction of CML and MAPK may become a new target in the treatment of CML.