SeO_2对原癌基因c-fos调控区P250的影响

Effects of Selenium Dioxide on Regulatory Regions P250 of c-fos Gene

背景与目的:已有研究表明,二氧化硒(SeO2)可诱导肺癌细胞株中c-fos蛋白高表达。本研究旨在通过研究SeO2对原癌基因c-fos调控区域P250的影响,探讨其调控机理。方法:构建c-fos启动区氯霉素乙酰化转移酶(chloramphenicolacetyl-transferase,CAT),然后转染HeLa癌细胞株,用不同浓度SeO2处理后观察细胞中CAT的活性。结果:不同浓度(3μmol/L、10μmol/L、30μmol/L)SeO2对转染了c-fosCATP250的HeLa癌细胞株作用后,其CAT的活性均增高,其积分光密度值分别为0.737±0.085、6.047±0.738、3.167±0.183,与对照组(0.490±0.046)相比,10μmol/L和30μmol/L浓度组的积分光密度值变化有统计学意义(P<0.05)。结论:SeO2可通过影响c-fos调控区域P250而发挥调控肿瘤细胞凋亡的生物学效应,SeO2可能具有潜在的抗肿瘤作用。

BACKGROUND & OBJECTIVE:This study was designed to investigate the impact of selenium dioxide (SeO2) on regulatory regions P250 of c fos gene and to seek possible regulation mechanism. METHODS:HeLa cells were transfected with plasmids containing upstream regulating regions of c fos chloramphenicol acetyl transferase (CAT). The cells were cultured in various concentration of selenium dioxide. CAT expression in transfected cells was observed. RESULTS:After transfected HeLa cells were exposed to selenium dioxide, CAT expression showed obvious increase, especially in 10 μmol/L and 30 μmol/L selenium dioxide group (P< 0 05). CONCLUSION:Trough affecting regulatory regions P250 of c fos gene, Selenium dioxide plays biological effect of regulating tumor cells. Selenium dioxide possibly has anti tumor effects.