胞核雄激素受体测定对评价前列腺癌、肝癌受体状态的意义

Significance of Both Nuclear and Cytosol Androgen Receptor (AR) in Assessment of AR Status in Prostate Carcinoma and Hepatocellular Carcinoma

背景与目的:雄激素受体(androgenreceptor,AR)与前列腺癌(prostatecarcinoma,PC)、原发性肝癌(hepatocellularcacinoma,HCC)的发生、发展有关,对临床治疗方案选择及预后的评估等有一定影响,准确判断肿瘤组织的AR状态有重要的临床意义。本研究旨在通过对胞核雄激素受体(nuclearAR,AnR)进行分析,探讨AnR对评估前列腺癌、原发性肝癌雄激素受体状态的意义。方法:取94例PC和192例HCC患者的肿瘤组织及癌周组织,采用受体放射配基结合分析法(radioligandbindingassay,RBA)对组织中胞浆雄激素受体(cytosolAR,AcR)、胞核雄激素受体的亲和力(affinity,KD)、最大结合容量(maximumconcentrationofreceptor,Bmax)进行分析。结果:PC患者中肿瘤组织AcR、AnR的Bmax值(58.82±34.73)、(543.70±249.44)fmol/mgprotein明显高于癌周组织的(21.63±14.89)、(89.20±47.32)fmol/mgprotein(P<0.001);其KD值(0.84±0.52)、(2.15±0.79)nmol/L与癌周组织的(0.78±0.49)、(2.24±0.84)nmol/L之间的差异无统计学意义(P>0.50)。HCC患者中,肿瘤组织AcR、AnR的Bmax值(18.09±16.87)、(59.93±34.12)fmol/mgprotein亦明显高于癌周组织的(10.87±7.60)、(25.54±20.10)fmol/mgprotein(P<0.001);其KD值(0.76±0.57)、(1.89±0.74)

BACKGROUND & OBJECTIVE: Androgen receptor (AR) is closely associated with the genesis,development,treatment and assessment of prognosis of prostate carcinoma (PC) and hepatocellular carcinoma (HCC).How to determine the AR status accurately has important clinical significance. This study was designed to investigate the significance of androgen receptor(AR) in cell nucleus for assessment of the AR status of patients with PC and HCC. METHODS: A total of 94 PC and 192 HCC tissues were analyzed for the affinity (KD),cytosol AR (AcR) and nuclear AR(AnR) using radioligand binding assay(RBA). RESULTS: In 94 PC tissues, the Bmax values of AcR and AnR were 58.82±34.73 and 543.70±249.44 fmol/mg protein which were significantly higher than those of the surrounding tissues (21.63±14.89 and 89.20±47.32 fmol/mg protein, P< 0.001).The KD values of AcR and AnR were 0.84±0.52 and 2.15±0.79 nmol/L which were no significantl difference as compared with the surrounding tissues(0.78±0.49 and 2.24±0.84 nmol/L, P >0.50) .In 192 HCC tissues, the Bmax values of AcR and AnR were 18.09±16.87 and 59.93±34.12 fmol/mg proterin, which were significantly higher than those of the surrounding tissues (10.87±7.60 and 25.54±20.10 fmol/mg protein, P< 0.001 ). The KD values of AcR and AnR were 0.76±0.57 and 1.89±0.74nmol/L)which were no significant difference as compared with the surrounding tissues(0.69±0.48 and 1.94±0.88 nmol/L, P >0.50),The ratio of AnR/AcR was also higher (P< 0.001). Of 94 PC tissues, 48.94% were both AcR and AnR positive, being lower than that of the tissues with positive AcR alone (77.66%).Of 192 HCC tissues, 34.09% were both AcR and AnR positive, being also lower than that of AcR positive alone (56.26%).However, 63.01% of PC and 62.03%of HCC AcR positive tissues were accompanied by AnR positive. CONCLUSION: Despite the fact that the Bmax of both AcR and AnR increased in PC and HCC tissues as compared to the surrounding tissues, the AnR showed more significant changes. In assessment of the AR status of PC and HCC tissues, it would be more accurate to analyze with both AcR and AnR than with AcR alone.