组织工程人工血管支架的预构

Fabrication of a blood vessel scaffold with a combined polymer for tissue engineering

目的 探讨具有血管平滑肌细胞 (vascularsmoothmusclecells,VSMCS)活性的组织工程人工血管支架的构建方法。方法 ①将聚羟基乙酸 (polyglycolicacid ,PGA)纤维无纺网支架材料、血管平滑肌细胞和胶原纤维相混合 ,构建组织工程血管。②将VSMCS 置入胶原凝胶中 ,观察VSMCS 的生长状况。③观察VSMCS 胶原悬液滴入该支架材料中的生长。结果 ①VSMCS 在胶原凝胶中的位置固定 ,分布于不同层次 ,约 3～ 4h逐渐形成多个胞质突起。随时间推移 ,胞质突起继续伸展 ,部分细胞呈梭形、纺锤形。②VSMCS 胶原悬液滴入胶原包埋处理的PGA支架中后 ,大部分细胞随胶原凝胶状态的形成 ,滞留于支架材料网孔中 ,细胞可沿PGA纤维表面生长。结论 胶原包埋处理的PGA纤维无纺网是良好的携带VSMCS 的多孔生物降解材料。

ObjectiveTo investigate the possibility to fabricate a blood vessel scaffold with a combined polymer for tissue engineering. Methods A blood vessel scaffold was designed with a combined polymer composed of rabbit vascular smooth muscle cells(VSMC-S),collagen and a non|spinning fabric mesh of polyglycolic acid (PGA).VSMC-S were implanted into collagen gel and their growth was observed.The mixed solution of VSMC-S and collagen was dropped into the tubular scaffold,followed by 7|day culturing. Results VSMC-S formed many prominences after culturing in gelatinous collagen for 3～4 hours.With cells extending,some cells became shuttle|or spindle|shaped.After VSMC-S|collagen complex was implanted into the PGA mesh,most of VSMC-S remained in the pore of PGA mesh with the formation of gelation.VSMC-S could adhere to and grow on the PGA fiber. ConclusionThe non|spinning PGA porous biodegradable material coated with collagen is a good carrier for VSMC-S to adhere and grow.;