结构蛋白及血管形成因子在体表海绵状静脉畸形中的表达及意义

The expression and significance of structural proteins, VEGF and Ang|1 in cavernous venous malformations of the body surface

目的 研究结构蛋白及血管形成因子 (VEGF)在体表海绵状静脉畸形 (cavernousvenousmalformation ,CVM)中的表达及意义。方法 1996～ 2 0 0 0年CVM病理样本 2 5例 ,取正常中、小型静脉各 12例。采用Envision法免疫组化染色观察Ⅳ型胶原、纤维连接蛋白 (Fn)、层粘连蛋白 (Ln)及VEGF、血管生成素 1(Ang 1)等血管形成因子的表达 ,半定量分析结果。结果 Ⅳ型胶原、Fn和Ln在海绵状静脉畸形与中、小静脉中的分布类似 ,但表达量明显较少。畸形组织和小静脉VEGF表达明显强于中型静脉 ,小静脉Ang 1表达明显强于静脉畸形和中型静脉。结论 Ln及VEGF表达变化可能是海绵状静脉畸形形成发展的重要因素。Ang 1表达减少可能参与海绵状静脉畸形的血管塑形障碍的发生。

ObjectiveTo study the expression and significance of structural proteins, VEGF and Ang|1 in cavernous venous malformations of the body surface. Methods Tissue samples came from 25 cases of cavernous venous malformations, 12 cases of normal moderate veins and 12 cases of normal small veins. Envision immunohistochemical stain was used to investigate the expression of Ⅳ collagen, fibronectin, laminin, VEGF and Ang|1. The results were analyzed semi|quantitatively. Results The distribution of structural proteins in cavernous venous malformations is similar to moderate and small veins, but the expression in venous malformations is less obviously. VEGF expression in cavernous venous malformations and small veins is stronger obviously than moderate veins. Ang|1 expression in small veins is stronger remarkably than cavernous venous malformations and moderate veins. ConclusionThe abnormal expression of structural proteins may be an important factor in etiopathology and progress of cavernous venous malformations. There is disturbance of blood vessel remodelling in the sinusoid of cavernous venous malformations, with which the less expression of Ang|1 may be related.;