Ⅱ型胶原口腔喷雾剂对小鼠胶原性关节炎的治疗作用(英文)

Effects of chicken typeⅡcollagen oral spraying on collagen-induced arthritis in mice

目的 观察鸡Ⅱ型胶原 (CⅡ )口腔喷雾剂对小鼠胶原性关节炎 (CIA)是否具有治疗作用及相关的机理。方法 建立小鼠CIA模型 ,观测关节积分变化。MTT法检测脾淋巴细胞增殖反应 ,腹腔巨噬细胞产生白介素 1(IL 1)及脾淋巴细胞产生白介素 2 (IL 2 )的活性。结果 CⅡ口腔喷雾剂 (5 ,10 ,2 0μg·kg- 1·d- 1× 10d ,免疫后d 2 5～d 35 )均能降低CIA小鼠关节积分 ,且作用与igCⅡ (10 μg·kg- 1·d- 1× 10d)及泼尼松龙 (2mg·kg- 1·d- 1× 10d)相当。口腔喷雾CⅡ (10 ,2 0 μg·kg- 1·d- 1× 10d)能抑制CIA小鼠刀豆蛋白A诱导的脾淋巴细胞增殖反应和IL 1、IL 2的产生活性。结论 CⅡ口腔喷雾剂能抑制CIA小鼠多发性关节炎 ,提示其对CIA小鼠有治疗作用。其机理可能与改善CIA小鼠异常的免疫功能有关。

AIM To examine whether chicken typeⅡcollagen (CⅡ) oral spraying has the therapeutic effect on the collagen-induced arthritis (CIA) in mice and investigate its related immunological mechanisms. METHODS CIA mouse model was established. The effect of treatment with CⅡspraying in mice was measured by paw-swelling score. Splenocytes proliferation, activity of interleukin-1 (IL-1) produced by peritoneal macrophages(PM) and interleukin-2 (IL-2) by splenocytes were assayed by MTT method. RESULTS CⅡ oral spraying(5, 10, 20 μg·kg -1 ·d -1 ×10 d, d 25-35 after immunization) decreased the mean of arthritis scores in CIA mice. The effect of CⅡ spraying was comparable with that of ig CⅡ and prednisone. Meanwhile, CⅡ oral spraying (10, 20 μg·kg -1 ·d -1 ×10 d) suppressed the ConA-induced splenocyte proliferation and the activity of IL-1 and IL-2 in CIA mice. CONCLUSION CⅡoral spraying suppresses the polyarthritis of CIA mice, which suggests that CⅡoral spraying have therapeutic effect on CIA mice. Its mechanism maybe related to the modification of the abnormal immunological function of CIA mice.