高效液相色谱法测定大鼠脑和NG108-15细胞内胍丁胺含量(英文)

Detection of endogenous agmatine content in rat brain and NG108-15 cells using high performance liquid chromatography

目的 建立测定大鼠不同脑区和NG10 8 15细胞内源性胍丁胺含量的方法。方法 用高效液相色谱荧光检测法测定大鼠不同脑区 (包括皮层、海马、丘脑、纹状体和小脑 )及NG10 8 15细胞内胍丁胺含量。结果 正常大鼠不同脑区胍丁胺的含量范围为 0 .8～ 1.71μg·g- 1湿脑组织 ;NG10 8 15细胞内含量为 0 .0 4 4mg·g- 1蛋白。结论 此法适于常规测定动物脑组织及培养细胞内的胍丁胺含量。

AIM To develop a method to determine endogenous agmatine(Agm) content in rat brains and cultured NG108-15 cells. METHODS High performance liquid chromatography(HPLC) with fluorescence detector was used to determine the endogenous Agm concentration in different regions (frontal cortex, thalamus, hippocampus, striatum and cerebellum) of rat brain and NG108-15 cells. RESULTS The concentration of endogenous Agm was 0.8-1.71 μg·g -1 wet tissues in different rat brain regions, and 0.044 mg·g -1 protein in NG108-15 cells, respectively. CONCLUSION This gradient HPLC procedure is applicable for routine detection of Agm in vertebrate tissues and cells.