不同药物对晚孕鼠子宫平滑肌及左心室心肌细胞电压依赖性钙通道-L亚型mRNA表达的影响

Effects of oxytocin,misoprostol and nimodiping on expression of L-type voltage dependant calcium channel mRNA of the uterine myometrium and left ventricular myocardial cells in late pregnant rats

目的 探讨缩宫素、米索前列醇及尼莫地平 3种药物对晚孕鼠子宫平滑肌与左心室心肌细胞电压依赖性钙通道 L亚型 (VDCC L)mRNA表达的影响。方法 应用半定量逆转录聚合酶链反应 (RT PCR)技术分别测定自然分娩鼠 (自然分娩组 )、缩宫素诱导分娩鼠 (缩宫素组 )、米索前列醇诱导分娩鼠 (米索前列醇组 )及尼莫地平作用 48h后分娩鼠 (尼莫地平组 )的子宫平滑肌与左心室心肌细胞VDCC Lα1、β2 亚单位mRNA的表达。结果 (1)自然分娩组、缩宫素组、米索前列醇组及尼莫地平组孕鼠的子宫平滑肌细胞VDCC Lα1mRNA的相对表达量依次为 0 .80 0 3± 0 .165 9、0 .863 1±0 .192 1、0 .812 0± 0 .173 4及 0 .742 6± 0 .182 6,缩宫素组与米索前列醇组虽高于自然分娩组及尼莫地平组 ,但 4组之间比较 ,差异均无显著性 (P >0 .0 5 ) ;VDCC Lβ2 mRNA的相对表达量依次为 0 .64 69± 0 .13 0 4、0 .5 0 62± 0 .1472、0 .5 0 0 5± 0 .13 5 6及 0 .492 9± 0 .12 76,自然分娩组虽高于其他用药的 3组 ,但差异无显著性 (P >0 .0 5 )。 (2 )自然分娩组、缩宫素组、米索前列醇组及尼莫地平组孕鼠的左心室心肌细胞VDCC Lα1mRNA相对表达量依次为 0 .662 5± 0 .180 1、0 .63 65± 0 .15 78、0 .5 917± 0 .1413及 0 .5 42

Objective To study the effect of oxytocin, misoprostol and nimodiping on expressions of L type voltage dependant calcium channel(VDCC L) α 1 and VDCC L β 2 mRNA in the myometrium and left ventricular myocardial cells of the late pregnant rats. Methods A reverse transcription polymerase chain reaction(RT PCR)method was used to examine and quantitate VDCC L α 1 and VDCC L β 2 mRNA expressions in the uterine myometrium and left ventricular myocardial cells of rats, which were divided into the nature labor group, oxytocin group (oxytocin inducing labor), misoprostol group (misoprostol inducing labor) and nimodipine group (active labor at 48 hours after administrating nimodipine). Results (1) In the uterine myometrium, the levels of VDCC L α1 mRNA expression in the nature labor, oxytocin, misoprostol and nimodipine group were 0.800 3±0.165 9, 0.863 1± 0.192 1 , 0.812 0± 0.173 4 and 0.742 6±0.182 6, respectively. No significant difference was found in the four groups ( P >0.05). The levels of VDCC L β 2 mRNA expression in the above four groups were 0.646 9±0.130 4,0.506 2±0.147 2, 0.500 5±0.135 6 and 0.492 9±0.127 6, respectively. There was no remarkable difference between the nature labor group and the other three administrating drugs groups ( P >0.05).(2) In the left ventricular myocardial cells,expressions of VDCC L α 1 in the nature labor, oxytocin, misoprostol and nimodipine group were 0.662 5± 0.180 1, 0.636 5±0.157 8, 0.591 7±0.141 3 and 0.542 6±0.143 6, respectively;the levels of VDCC L β 2 mRNA expression were 0.670 2±0.140 5, 0.606 2±0.143 9, 0.591 4±0.121 9 and 0.585 2±0.131 0, respectively. Although both the levels of VDCC L α 1 and VDCC L β 2 mRNA expression in the nature labor group were a little higher than those in the other three experiment groups, the statistic difference was not noted( P >0.05). Conclusions Oxytocin, misoprostol or nimodipine can induce or inhibit labor through regulating expressions of VDCC L α 1 and VDCC L β 2 mRNA in the rat uterine myometrium and it may not have an adverse effect on heart function of normal pregnant rats. VDCC L may be the common channel of labor induced by internal or external factors.