摘要
将中药方 1、方 2、和方 3的粗提物与鸡传染性法氏囊病毒以三种顺序 (先加中药后加病毒、先加病毒后加中药、中药和病毒同时加入 )加入到培养 2 4h、已长成完整单层的鸡胚成纤维细胞 (CEF)上 ,观察它们对病毒感染细胞能力的影响。结果表明 :在细胞安全浓度范围内 ,方 1、方 2和方 3的粗提物在先于病毒或与病毒同时加入时均能抑制病毒感染 ,且与浓度和加药方式有关 ;以补气、清热凉血、滋阴为主的方 3优于以补气、清热凉血为主的方 1和以补气为主的方 2。
Three kinds of crude extracts from Chinese herbal medicinal prescriptions were respectively added onto monolayer of chick embryo fibroblast(CEF)cultured 24 hours as the followed methods:before,after and simultaneity with IBDV together.The effects of these extracts on cellular infectivity of the IBDV were observed.The results showed that all of three kinds of crude extracts under toxic concentrations did not inhibit IBDV only when added earlier than and simultaneously with IBDV,but also be influenced by their concentration and the methods of adding medicine.Effect of prescrption 3 characterized by energen-invigorating,antipyretic,blood-cooling and Yin-nourishing is better than prescription 1 characterized by energeninvigorate,antipyretic and blood-cooling presctription 2 characterized by energen-invigorating
出处
《中兽医医药杂志》
2003年第1期8-10,共3页
Journal of Traditional Chinese Veterinary Medicine
基金
江苏生物制品工程技术研究中心开放实验开放课题
