灵芝原生质体诱变筛选富硒高生物量菌株

Mutagenesis of Ganoderma lucidum protoplasts for high rate of biomass production and Se^(+4)-rich isolates.

研究了灵芝(Ganodermalucidum)CJXN-9802的原生质体形成和影响原生质再生的各因素,其中包括酶,温度,渗透压调节剂和菌龄等。并对灵芝原生质体进行了紫外照射诱变,筛选获得了快速生长的诱变菌株。诱变株之一的灵芝CJXN-980222菌株硒含量有很大的提高,比出发株提高9.4%,生物量比出发株高14.3%。

Protoplasts were isolated from Ganoderma lucidum CJXN9802. Factors including enzymes, hypha age, osmotants, and temperature were studied with respect their influence on protoplast production. Rapid growth mutants were obtained through screening from UVlight irradiated protoplasts. One of the mutants CJXN980222 showed increase in biomass 9.4% and 14.3% increase in Se+4 content, compared with the original strain.