摘要
To setup a method of amplification for the whole Cag A gene of H elicobacter pylori and its fingerprinting by restriction fragm entlength polym orphism(RFL P) ,nested PCR was employed in com bination with TD- PCR to am plify the gene and Eco RΙ and Hind were used to generate the RFL P fingerprinting.Target DNA fragm ents from 13of2 0 samples were successfully amplified and the relevant RFL P fingerprintings were obtained.It is concluded thatthe m ethod can be used to amplify the whole Cag A gene and Cag A gene has apparent diversity of RFL P profile.
To setup a method of amplification for the whole Cag A gene of H elicobacter pylori and its fingerprinting by restriction fragm entlength polym orphism(RFL P) ,nested PCR was employed in com bination with TD- PCR to am plify the gene and Eco RΙ and Hind were used to generate the RFL P fingerprinting.Target DNA fragm ents from 13of2 0 samples were successfully amplified and the relevant RFL P fingerprintings were obtained.It is concluded thatthe m ethod can be used to amplify the whole Cag A gene and Cag A gene has apparent diversity of RFL P profile.
基金
Thisprojectwassupported by a grant from the MinistryofPublicHealth(Serial No.:98- 1- 12 3)
