毛细管气相色谱法测定血中的丁卡因

Analysis of Amethocaine in Whole Blood Using Capillary Gas Chromatography

本文报道应用毛细管色谱柱和氮磷检测器的气相色谱法,测定了全血中局部麻醉药丁卡因。选用SKF_(525A)为内标物,对前处理过程进行质量控制。全血碱化后,用乙醚提取。丁卡因在10～80ng/μl范围内呈线性关系,r=0.999。丁卡因回收率为74.7%,C.V6.09%;内标物SKF_(525A)回收率为70.3%,C.V3.32%。本文建立的方法可用于法庭毒物分析及临床分析。

This report describes a senstive capillary gas chromatographi method with the use of a nitrogen detector for determination of Amethocaine in whole blood. Amethocaine is local anqe- sthesia. SKF525A was used for internal standard (I.S). Diether were extraction solvent. The calibracion cure rang of amethocine were 10～80 ng/ul(r=0. 999) .The average recovery of Amethocaine and I. S were 74.7% and 70. 3%, CV(%) were 6. 09 and 3. 32(n=5). This method applies to forensic toxicology analysis and clinic analysis.