农药生物传感器所用酶的比较研究

Study on Esterase Used in Biosensor for Pesticide Residue Assay

在利用农药对酶的抑制反应来检测农药的方法中,选用的酶既可以是动物酯酶,也可以是植物酯酶。本文采用分光光度计法,对植物酯酶和动物酯酶在不同pH值下的酶活、抑制程度等性质进行了实验研究。结果表明:动物酯酶中的苍蝇酶、蝇蛆酶与植物酯酶中的小麦酯酶相比,它们的活力和被农药抑制程度处于相近的水平;植物酯酶中小麦与玉米相比,玉米酯酶的活力远低于小麦酯酶的活力,不适于用来进行抑制反应来检测农药。动物酯酶在pH值为7.5时活力最高,小麦酯酶在pH值6.5时最高。

Bothanimal -esterase and plant -esterase can be inhibited by pesticide.So,plant -esterase,as well as animal -esterase,can be used to detect pesticide residue.In this paper,plant -esterases,compared with animal -esterase were studied on activity,inhibition degree and relate d properties.The results of experim ent showed that the activity of plant -esterases and inhibition degree on pla nt -esterases was almost the same as an-imal -esterases.Both as plant -este rase,corn esterase' s activity was much lower than wheat' s.So it was not suitable to detect pesticide by usin g corn esterase.The pH effect was als o studied.The optimal pH value for plant -esterase was 6.5,and that for animal -esterase was 7.5.