摘要
建立并优化了转基因大豆与玉米的DNA提取方法,针对CaMV35S启动子和T-NOS终止子的序列特点设计特异性引物与探针,应用PCR-ELISA检测技术,建立了转基因大豆与玉米中常用外源基因的快速检测体系,并应用于进出境产品的转基因检测实际工作中。结果表明,建立的PCR-ELISA法具有操作简便、灵敏特异、结果准确的优点,可对转基因大豆、玉米及其它转基因产品进行定性和定量检测。
The methods of extracting DNAfromgenetically modified organisms (GMOs )were studied and ob-timized.According to the characteristics of CaMV 35S promoter&T -NOS te rminator introduced into GMOs ,PCR -enzyme linked immunosorbent assay(PCR -ELISA)methods were suitable for screening of transgenic elements andtherefore established.Withthis methodwe analyzedsoybeans andcorns.The results showedthatthemethods of PCR -ELISAwere simple,rapid and reliable for qualitative a nd quantificational analysis of GMOs.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2003年第1期101-105,共5页
Food Science
基金
厦门市科技计划资助项目(3502Z2001109)