期刊文献+

应用PCR-ELISA技术检测转基因产品的研究 被引量:16

Study on PCR-ELISA for the Detection of Genetically Modified Organisms Products
下载PDF
导出
摘要 建立并优化了转基因大豆与玉米的DNA提取方法,针对CaMV35S启动子和T-NOS终止子的序列特点设计特异性引物与探针,应用PCR-ELISA检测技术,建立了转基因大豆与玉米中常用外源基因的快速检测体系,并应用于进出境产品的转基因检测实际工作中。结果表明,建立的PCR-ELISA法具有操作简便、灵敏特异、结果准确的优点,可对转基因大豆、玉米及其它转基因产品进行定性和定量检测。 The methods of extracting DNAfromgenetically modified organisms (GMOs )were studied and ob-timized.According to the characteristics of CaMV 35S promoter&T -NOS te rminator introduced into GMOs ,PCR -enzyme linked immunosorbent assay(PCR -ELISA)methods were suitable for screening of transgenic elements andtherefore established.Withthis methodwe analyzedsoybeans andcorns.The results showedthatthemethods of PCR -ELISAwere simple,rapid and reliable for qualitative a nd quantificational analysis of GMOs.
出处 《食品科学》 EI CAS CSCD 北大核心 2003年第1期101-105,共5页 Food Science
基金 厦门市科技计划资助项目(3502Z2001109)
关键词 转基因产品 检测技术 PCR-ELISA法 大豆 玉米 GMOs detection technique PCR -ELI SA
  • 引文网络
  • 相关文献

参考文献2

二级参考文献24

  • 1-.多国通过基因食物贸易协议[J].食品工业科技,2000,21(3):1-1.
  • 2http//www.isaaa.org/frbrief8.html
  • 3Soryu N.Transformation of cucumber plant using Agrobacterium tume faciens and regeneration from hypocotylex plants[J].Plant Cell Report,1996,15:809-814.
  • 4Dong J Z,Yang M Z,Jia S R,et al.Transformation of melon and expression from the cauliflower mosaic virus 35s promoter in transgenic melon plant [J].Biotech,1991,9:858-863.
  • 5Bryant J,Leather S.Removal of selectable marker genes from transgenic plants:needless sophistication or social necessity [J].Trends in Biotech,1992,10:274-275.
  • 6Ioan N,Stefan H,Danielle C,et al.Plant protoplasts as genetic tool:selectable marker for developmental studies [J].Int Dev Biol,1992,36:73-84.
  • 7Sullivann J.Transformation of liquid mbar string raciflua using Agrobacterium tume faciens [J].Plant Cell Report,1993,12:303-306.
  • 8Ow DW.Transient and stable expression of the firefly luciferase gene in plant cells and transgenic plants [J].Science,1986,234:856-859.
  • 9Deblaere R.Efficient Octopine Ti.plasmid derived vectors for Agrobacterium-mediated gene transfer to plant [J].Nucl Acid Res,1985,13:4777-4788.
  • 10Chyi,Yan-san R,Jorgensen A,et al.Location and stability of Agrobacterium-mediated T-DNA insertions in the Lycopersicon genome [J].Mol Gen Genet,1986,204:64-69.

共引文献46

同被引文献231

引证文献16

二级引证文献67

;
使用帮助 返回顶部