摘要
提出了用Ni(Ⅱ)处理10μm强酸型阳离子交换剂Partisil-10SCX再吸附葡萄糖氧化酶以制备固定化酶的新方法。酶的吸附和固定化酶的活性较一般的物理吸附有明显的改善。由此制得的固定化酶柱后反应器接入微型高效液相色谱-电化学检测体系进行了血清和全血中葡萄糖含量的测定,并实现了葡萄糖和尿酸的同时检测。
Glucose in serum and whole blood can be determined by system of micro-HPLC-immobilizedenzyme post column reactor which was specially prepared by adsorption of glucose oxidase onNi(Ⅱ) pretreated Partisil--10SCX (a kind of 10μm strong cation exchanger)-electrochemical de-tection, by this method glucose and uric acid can be determined simutaneously.
基金
国家自然科学基金
关键词
固定化酶
柱后反应器
葡萄糖
血清
Immobilized enzyme
Post column reactor
Glucose oxidase
Glucose
Serum
