参附汤上调HL60细胞热休克蛋白和糖皮质激素受体mRNA表达及其对细胞存活的影响

Effect of Shenfutang in Up-regulating Expressions of Heat Shock Protein mRNA and Glucocorticoid Receptor mRNA and Its Influence on Cell Survival

目的 :探讨中药方剂参附汤对HL6 0细胞热休克反应时热休克蛋白信使核糖核酸 (heatshockproteinmRNA ,HSPmRNA)和糖皮质激素受体信使核糖核酸 (glucocorticoidreceptormRNA ,GRmRNA)表达及对细胞存活率的影响。方法 :建立细胞热休克模型 ,用动物血清学方法制备参附汤 (SFT)血清 ,用Northernblot分析HSPmRNA和GRmRNA的改变 ,用台盼蓝拒染法观察细胞存活率。结果 :HL6 0细胞热休克反应时GRmRNA明显减少 ,细胞存活率下降 ,HSPmRNA则增加 ;该细胞经SFT处理后在热休克反应时 ,GRmRNA表达增加 ,细胞存活率提高 ,HSPmRNA进一步增加。结论 :SFT可上调HL6 0细胞热休克反应时HSPmRNA和GRmRNA表达 ,提高糖皮质激素的生物学效应和抗细胞损伤能力 ,从而提高HL6 0细胞在热休克时的存活率 ,这可能是临床上SFT用于治疗失血性休克的机制之一。

Objective: To explore the effect of Shenfutang (SFT) in up regulating expressions of heat shock protein (HSP) mRNA and glucocorticoid receptor (GR)mRNA and its influence on cell survival. Methods: HSP cell model was established. SFT containing serum was prepared with animal serologic method. Changes of HSPmRNA and GRmRNA were analyzed by Northern blot and cell survival rate was determined by Trypan blue staining test. Results: During HSP reaction, GRmRNA decreased obviously, cell survival rate lowered and HSPmRNA increased in HL60 cell line, while in the cell line being treated with SFT, during HSP reaction, GRmRNA and cell survival rate increased and HSPmRNA increased further. Conclusion: SFT could up regulate the expressions of HSPmRNA and GRmRNA in HL60 cell line, raise the biological activity and injure antagonizing capacity of glucocorticoid, thus to increase the survival rate of HL60 cell line during heat shock reaction. These may be the mechanism of SFT therapy used in clinical practice.